caa a22 4500 445323450 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0246-4 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0246-4 Genetic transformation of lipid transfer protein encoding gene in Phalaenopsis amabilis to enhance cold resistance [Elektronische Daten] [Xuyin Qin, Yang Liu, Shanjing Mao, Tianbi Li, Hsinkan Wu, Chengcai Chu, Youping Wang] Embryogenic callus of Phalaenopsis amabilis derived from leaf tissue was cocultivated with Agrobacterium tumefaciens strain LBA4404 harboring a plant cloning vector. The vector carried the lipid transfer protein (LTP) encoding gene cloned from cold tolerant Brazilian upland rice cv. IAPAR 9. The highest transformation efficiency (12.16%) was obtained when 1-2mm calli were infected and cocultivated with 0.4 (OD600) A. tumefaciens for 20min. Transgene integration of kan-resistant plants was confirmed through polymerase chain reaction analysis and Southern hybridization. Four hundred seventy transgenic plants, each derived from an independent protocorm-like body, were obtained. The expression of rice cold-inducible LTP gene in transgenic P. amabilis improved its adaptive responses to cold stress. The examination of transgenic plants revealed that enhanced cold tolerance was most likely due to the increased accumulation of several compatible solutes such as total soluble sugars, proline, antioxidant superoxide dismutase, decreased accumulation of malondialdehyde, and maintained electrolytes within the membrane compared with controls. Springer Science+Business Media B.V., 2010 Phalaenopsis amabilis nationallicence Agrobacterium tumefaciens -mediated transformation nationallicence LTP gene nationallicence Cold tolerance nationallicence ANOVA : Analysis of variance nationallicence AS : Acetosyringone nationallicence BAP : 6-Benzylaminopurine nationallicence Cef : Cefotaxime nationallicence EDTA : Ethylene diaminetetraacetic acid nationallicence EL : Electrolyte leakage nationallicence Kan : Kanamycin nationallicence LB : Lauria bertani nationallicence LTP : Lipid transfer protein nationallicence MDA : Malondialdehyde nationallicence MET : Methionine nationallicence MS : Murashige and Skoog medium nationallicence NAA : Naphthalene acetic acid nationallicence NBT : Nitroblue tetrazolium nationallicence npt II : Neomycin phosphotransferase II nationallicence PCR : Polymerase chain reaction nationallicence PLB : Protocorm-like body nationallicence pUbi : Plant ubiquitin promoter nationallicence SDW : Sterile distilled water nationallicence SDS : Sodium dodecyl sulfate nationallicence SOD : Superoxide dismutase nationallicence TBA : Thiobarbituric acid nationallicence TDZ : Thidiazuron nationallicence TE : Transformation efficiency nationallicence VB6 : Pyridoxine HCl nationallicence Qin Xuyin College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Liu Yang College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Mao Shanjing College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Li Tianbi College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Wu Hsinkan Yangping Horticulture Co. Ltd, 225008, Yangzhou, China aut Chu Chengcai Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Wang Youping College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Euphytica Springer Netherlands 177/1(2011-01-01), 33-43 0014-2336 177:1<33 2011 177 10681 https://doi.org/10.1007/s10681-010-0246-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0246-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Xuyin College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Liu Yang College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Mao Shanjing College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Li Tianbi College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Wu Hsinkan Yangping Horticulture Co. Ltd, 225008, Yangzhou, China aut NATIONALLICENCE 700 1- Chu Chengcai Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wang Youping College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 33-43 0014-2336 177:1<33 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer