caa a22 4500 445323469 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10681-010-0249-1 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0249-1 Transgenic fertile soybean plants derived from somatic embryos transformed via the combined DNA-free particle bombardment and Agrobacterium system [Elektronische Daten] [Beatriz Wiebke-Strohm, Annette Droste, Giancarlo Pasquali, Marina Osorio, Lauro Bücker-Neto, Luciane Passaglia, Marta Bencke, Milena Homrich, Márcia Margis-Pinheiro, Maria Bodanese-Zanettini] An Agrobacterium-mediated transformation procedure for soybean [Glycine max L. Merrill] proliferating somatic embryos is here described. The Agrobacterium tumefaciens LBA4404 strain harboring pTOK233, pCAMBIA1390-olp or pH7WG2Dwrky plasmids was used to mediate gene transfer into the plant genome. Prior to Agrobacterium inoculation, proliferative soybean embryogenic clusters were microwounded by DNA-free tungsten particle bombardment. Three independent transformation experiments were performed. In Experiment I, 26 transgenic plants were obtained from a unique clone of cv Bragg, while 580 plants were recovered from 105 clones of cv IAS5. In Experiment II, a single hygromycin-resistant clone of cv BRSMG68 Vencedora was recovered and gave rise to five plants. In Experiment III, 19 plants of cv Bragg and 48 plants of IAS5 were recovered, representing five and 14 independent transformation events, respectively. PCR and Southern analyses confirmed the transgenes' integration into plant genomes. Transgenic plants were fertile. They flowered, set pods and seeds. Transgene segregation in two T1 progenies fits the Mendelian pattern (3:1 transgenic:non-transgenic plants). This is the first report of transgenic fertile soybean plants obtained from somatic embryogenic tissues transformed by the system that combines DNA-free particle bombardment and Agrobacterium. Springer Science+Business Media B.V., 2010 Agrobacterium tumefaciens nationallicence Embryogenic tissues nationallicence Genetic transformation nationallicence Glycine max nationallicence Somatic embryogenesis nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence cv : Cultivar nationallicence PCR : Polymerase chain reaction nationallicence Wiebke-Strohm Beatriz Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Droste Annette Universidade do Vale do Rio dos Sinos, São Leopoldo, RS, Brazil aut Pasquali Giancarlo Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Osorio Marina Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Bücker-Neto Lauro Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Passaglia Luciane Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Bencke Marta Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Homrich Milena Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Margis-Pinheiro Márcia Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Bodanese-Zanettini Maria Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut Euphytica Springer Netherlands 177/3(2011-02-01), 343-354 0014-2336 177:3<343 2011 177 10681 https://doi.org/10.1007/s10681-010-0249-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0249-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wiebke-Strohm Beatriz Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Droste Annette Universidade do Vale do Rio dos Sinos, São Leopoldo, RS, Brazil aut NATIONALLICENCE 700 1- Pasquali Giancarlo Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Osorio Marina Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Bücker-Neto Lauro Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Passaglia Luciane Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Bencke Marta Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Homrich Milena Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Margis-Pinheiro Márcia Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Bodanese-Zanettini Maria Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, CP 15053, 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/3(2011-02-01), 343-354 0014-2336 177:3<343 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer