caa a22 4500 445323477 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10681-010-0264-2 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0264-2 Expression of Bruguiera gymnorhiza BgARP1 enhances salt tolerance in transgenic Arabidopsis plants [Elektronische Daten] [Masashi Miyama, Yuichi Tada] We have previously reported that expression of salt-responsive genes, including Bruguiera gymnorhiza ankyrin repeat protein 1 (BgARP1), enhances salt tolerance in both Agrobacterium tumefaciens and Arabidopsis. In this report, we further characterized BgARP1-expressing Arabidopsis to elucidate the role of BgARP1 in salt tolerance. BgARP1-expressing plants exhibited more vigorous growth than wild-type plants on MS plates containing 125-175mM NaCl. Real-time PCR analysis showed enhanced induction of osmotin34 in the 2-week-old transformants under 125mM NaCl. It was also showed that induction of typical salt-responsive genes, including RD29A, RD29B, and RD22, was blunted and delayed in the 4-week-old transformants during 24h after 200mM NaCl treatment. Ion content analysis showed that transgenic plants contained more K+, Ca2+, and NO3 −, and less NH4 +, than wild-type plants grown in 200mM NaCl. Our results suggest that BgARP1-expressing plants may reduce salt stress by up-regulating osmotin34 gene expression and maintaining K+ homeostasis and regulating Ca2+ content. These results indicate that BgARP1 is functional on a heterogeneous background. Springer Science+Business Media B.V., 2010 Bruguiera gymnorhiza nationallicence Salt tolerance nationallicence BgARP1 nationallicence Ankyrin repeat protein nationallicence Transgenic Arabidopsis nationallicence Mangrove nationallicence Miyama Masashi School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, 192-0982, Hachioji, Tokyo, Japan aut Tada Yuichi School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, 192-0982, Hachioji, Tokyo, Japan aut Euphytica Springer Netherlands 177/3(2011-02-01), 383-392 0014-2336 177:3<383 2011 177 10681 https://doi.org/10.1007/s10681-010-0264-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0264-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyama Masashi School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, 192-0982, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Tada Yuichi School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, 192-0982, Hachioji, Tokyo, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/3(2011-02-01), 383-392 0014-2336 177:3<383 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer