caa a22 4500 44532354X CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10681-010-0278-9 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0278-9 Semi-dominant genes confer additive tolerance to metribuzin in narrow-leafed lupin ( Lupinus angustifolius L.) mutants [Elektronische Daten] [Ping Si, Gang Pan, Mark Sweetingham] Narrow-leafed lupin (Lupinus angustifolius L.) is a grain legume well-adapted to sandy acid soils in a Mediterranean climate. Improved metribuzin tolerance in lupin cultivars is considered essential to protect crops from herbicide damage in Australia. This paper reports on the inheritance of metribuzin tolerance in two induced mutants Tanjil-AZ-33 and Tanjil-AZ-55 over the susceptible wild type cv. Tanjil. Both mutants were highly tolerant to 800g/ha metribuzin with no foliage damage, but cv. Tanjil died and reciprocal F1 hybrids had intermediate tolerance with foliage damage. The F2 populations of both crosses, Tanjil-AZ-33×Tanjil and Tanjil-AZ-55×Tanjil, had a segregation ratio of 1:2:1 for highly tolerant: damaged:dead plants. Progeny tests (F3) of selected F2 single plants confirmed that highly tolerant F2 plants were homozygous and damaged F2 plants were heterozygous. Clearly a single semi-dominant gene conferred metribuzin tolerance in both mutants. An allelism test revealed that the two mutants had two non-allelic tolerance genes with F2 plants segregating in a 15:1 ratio for survival and death at 800g/ha metribuzin. The tolerance gene in Tanjil-AZ-33 was designated as Mt3 and the gene in Tanjil-AZ-55 as Mt5. At 4,000g/ha metribuzin, 1/16 of F2 plants from the cross between the two mutants had no herbicide damage, suggesting the additive effects of the two tolerance genes, whilst the rest were damaged or dead. Combining these two tolerance genes, Mt3 and Mt5, increased tolerance further by approximately five-fold. Springer Science+Business Media B.V., 2010 Additive gene effect nationallicence Allelism test nationallicence Induced mutant nationallicence Metribuzin tolerance nationallicence Narrow-leafed lupin nationallicence Non-allelic genes nationallicence Semi-dominant nationallicence Si Ping Centre for Legumes in Mediterranean Agriculture (CLIMA), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, 6009, Crawley, WA, Australia aut Pan Gang Centre for Legumes in Mediterranean Agriculture (CLIMA), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, 6009, Crawley, WA, Australia aut Sweetingham Mark Western Australian Department of Agriculture and Food, Bentley Delivery Centre, Locked Bag No. 4, 6983, Bentley, WA, Australia aut Euphytica Springer Netherlands 177/3(2011-02-01), 411-418 0014-2336 177:3<411 2011 177 10681 https://doi.org/10.1007/s10681-010-0278-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0278-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Si Ping Centre for Legumes in Mediterranean Agriculture (CLIMA), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, 6009, Crawley, WA, Australia aut NATIONALLICENCE 700 1- Pan Gang Centre for Legumes in Mediterranean Agriculture (CLIMA), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, 6009, Crawley, WA, Australia aut NATIONALLICENCE 700 1- Sweetingham Mark Western Australian Department of Agriculture and Food, Bentley Delivery Centre, Locked Bag No. 4, 6983, Bentley, WA, Australia aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/3(2011-02-01), 411-418 0014-2336 177:3<411 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer