caa a22 4500 445323558 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10681-010-0262-4 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0262-4 Transgenic soybean with low phytate content constructed by Agrobacterium transformation and pollen-tube pathway [Elektronische Daten] [Shaohui Yang, Guilan Li, Minggang Li, Jiehua Wang] The expression of a microbial phytase in transgenic plants may create a new biochemical pathway that mobilizes its endogenous phytate and release inorganic phosphate from it, so that more phosphorus is available for plant growth. In this study, transgenic soybean plants were generated via both Agrobacterium transformation and pollen tube pathway with the PhyA gene of Aspergillus ficuum. The optimal concentrations of plant hormones including N6-benzylaminopurine (BAP), gibberellin (GA3) and indole-3-butyric acid (IBA) were tested based on their effectiveness on promoting the growth of transgenic explants. Genomic PCR results and Southern blot hybridization analysis showed that transgenic soybean plants selected for resistance to kanamycin contained the phyA transgene. The transgenic soybean plants with phyA gene integrated in their genome exhibited lower amount of phytate in different soybean tissues including leaf, stem and root, which indicated that engineering crop plants with a higher expression level of heterologous phytase could improve the degradation of phytate and potentially in turn mobilize more inorganic phosphate from phytate and thus reduce phosphate load on agricultural ecosystems. Springer Science+Business Media B.V., 2010 Microbial phytase nationallicence Phytate nationallicence Transgenic soybean nationallicence Agrobacterium transformation nationallicence Pollen tube pathway nationallicence Yang Shaohui School of Agriculture and Bioengineering, Tianjin University, 300072, Tianjin, People's Republic of China aut Li Guilan Hebei Normal University of Science & Technology, 066600, Changli, People's Republic of China aut Li Minggang College of Life Sciences, Nankai University, 300071, Tianjin, People's Republic of China aut Wang Jiehua School of Agriculture and Bioengineering, Tianjin University, 300072, Tianjin, People's Republic of China aut Euphytica Springer Netherlands 177/3(2011-02-01), 375-382 0014-2336 177:3<375 2011 177 10681 https://doi.org/10.1007/s10681-010-0262-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0262-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Shaohui School of Agriculture and Bioengineering, Tianjin University, 300072, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Li Guilan Hebei Normal University of Science & Technology, 066600, Changli, People's Republic of China aut NATIONALLICENCE 700 1- Li Minggang College of Life Sciences, Nankai University, 300071, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jiehua School of Agriculture and Bioengineering, Tianjin University, 300072, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/3(2011-02-01), 375-382 0014-2336 177:3<375 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer