caa a22 4500 445323566 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10681-010-0251-7 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0251-7 The development of a co-dominant marker for the β-conglycinin α′ subunit in soybeans [Elektronische Daten] [Sung-Il Kim, Moon Kim, Kyujung Van, Yeong-Ho Lee, Hong Kim, Chun Cai, Bong-Soo Park, Hak-Soo Seo, Suk-Ha Lee] The development of soybean varieties that lack the β-conglycinin α′ subunit is an attractive goal because the β-conglycinin α′ subunit exerts a negative influence on nutrition and tofu gelation, and is also a major allergen. We sought to develop a co-dominant DNA marker for the β-conglycinin α′ subunit (Cgy-1) gene for use in marker-assisted selection (MAS). We identified a deleted sequence responsible for the null allele of Cgy-1 in a soybean variety lacking the β-conglycinin α′ subunit known as ‘Keburi'. The deletion spanned 12,998bp and included Cgy-1 and its incomplete tandem duplicate on chromosome 10. Based on the Cgy-1 sequence from both the Williams 82 soybean reference sequence and the Keburi variety, a set of three allele-specific primers was designed for multiplex PCR assay. These primers enabled allelic discrimination by the sizes of PCR products. This amplified two distinct DNA fragments of 913 and 649bp in Williams 82 and Keburi, respectively. The practicality of the developed co-dominant marker for Cgy-1 was also confirmed by amplification in five other soybean varieties including three wild types and two mutants. The heterozygosity of the F1 plants at the Cgy-1 locus was ascertained using our novel co-dominant marker. This PCR-based co-dominant marker is capable of detecting the presence or absence of β-conglycinin α′ subunit for soybean marker assisted breeding system. Springer Science+Business Media B.V., 2010 β-Conglycinin α′ subunit nationallicence Cgy - 1 nationallicence Co-dominant marker nationallicence Seed storage protein nationallicence Soybean nationallicence Kim Sung-Il Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Kim Moon Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Van Kyujung Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Lee Yeong-Ho Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Kim Hong Department of Crop Science, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut Cai Chun College of Life Science, Qingdao Agricultural University, 266109, Qingdao, China aut Park Bong-Soo Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Seo Hak-Soo Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Lee Suk-Ha Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut Euphytica Springer Netherlands 177/3(2011-02-01), 355-363 0014-2336 177:3<355 2011 177 10681 https://doi.org/10.1007/s10681-010-0251-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0251-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Sung-Il Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Moon Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Van Kyujung Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Yeong-Ho Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Hong Department of Crop Science, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut NATIONALLICENCE 700 1- Cai Chun College of Life Science, Qingdao Agricultural University, 266109, Qingdao, China aut NATIONALLICENCE 700 1- Park Bong-Soo Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Seo Hak-Soo Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Suk-Ha Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Korea aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/3(2011-02-01), 355-363 0014-2336 177:3<355 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer