caa a22 4500 445323698 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0263-3 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0263-3 Enhancement of drought resistance and biomass by increasing the amount of glycine betaine in wheat seedlings [Elektronische Daten] [Chunmei He, Weiwei Zhang, Qiang Gao, Aifang Yang, Xiaorui Hu, Juren Zhang] Drought is a major agricultural menace reducing crop productivity and limiting the successful realization of land potential throughout the world. Therefore, breeding common wheat with improved drought-tolerance via genetic manipulation is of great importance. We have introduced the betA gene encoding choline dehydrogenase from Escherichia coli into common wheat (Triticum aestivum L.) by Agrobacterium-mediated transformation. Various levels of expression of the betA gene were confirmed by RT-PCR among the transgenic lines and different levels of glycine betaine accumulation were detected in these lines. Several wheat transgenic lines with different betA expression levels in the T3 generation and wild-type (WT) were selected to test their performance under drought stress conditions. Water deficit in plants caused a reduction in photosynthesis and activity of the PSII complex and resulted in increased accumulation of osmolytes. Drought stress also led to lower membrane stability along with much higher activities of superoxide dismutase and peroxidase in all wheat lines. However, wheat lines that were transgenic for the betA gene were less injured and exhibited greater root length and growth compared with the WT. It was concluded that the amount of injury to the wheat plants was negatively correlated with the level of accumulation of glycine betaine, and the glycine betaine acted as an important osmoprotectant in transgenic plants to improve root growth, and enhance the resistance of transgenic plants to drought stress. Springer Science+Business Media B.V., 2010 Wheat ( Triticum aestivum L.) nationallicence Transgene nationallicence Drought stress nationallicence Glycine betaine nationallicence BADH : Betaine aldehyde dehydrogenase nationallicence CDH : Choline dehydrogenase nationallicence CMO : Choline monooxygenase nationallicence GB : Glycine betaine nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence MDA : Malondialdehyde nationallicence RWC : Relative water content nationallicence Ls : Stomatal limitation value nationallicence NBT : Nitroblue tetrazolium nationallicence POD : Peroxidase nationallicence SOD : Superoxide dismutase nationallicence ROS : Reactive oxygen species nationallicence WT : Wild-type nationallicence He Chunmei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Zhang Weiwei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Gao Qiang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Yang Aifang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Hu Xiaorui School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Zhang Juren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Euphytica Springer Netherlands 177/2(2011-01-01), 151-167 0014-2336 177:2<151 2011 177 10681 https://doi.org/10.1007/s10681-010-0263-3 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0263-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Chunmei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Weiwei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Gao Qiang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Yang Aifang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xiaorui School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Juren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/2(2011-01-01), 151-167 0014-2336 177:2<151 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer