caa a22 4500 445323779 CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10681-010-0279-8 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0279-8 Pyramiding of two bacterial blight resistance and a semidwarfing gene in Type 3 Basmati using marker-assisted selection [Elektronische Daten] [Deepak Rajpurohit, Rahul Kumar, Mankesh Kumar, Priyanka Paul, Anjali Awasthi, P. Osman Basha, Anju Puri, Tripta Jhang, Kuldeep Singh, Harcharan Dhaliwal] A traditional Type 3 Basmati rice cultivar grown in India is tall and lodges even under low nitrogen fertilizer dose. In addition to lodging, it is highly susceptible to several diseases and pests including bacterial blight (BB). BB resistance genes (Xa21 and xa13) and a semidwarfing gene (sd-1) were pyramided in Type 3 Basmati from a rice cultivar PR106-P2 using marker-assisted selection (MAS). Foreground selection for BB resistance genes, Xa21 and xa13 and reduced plant height gene, sd-1 was carried on the basis of linked molecular markers pTA248, RG136 and ‘h', respectively. The BC2F3 progenies with both the BB resistance genes were highly resistant with lower lesion length than either of the genes individually. Background profiling of the selected 16 BC2F3 progenies was done using 95 anchored SSR and 12 ISSR markers. Among the selected 16 BC2F3 progenies, 38-5-2 and 38-5-36 closely clustered along with the recipient parent Type 3 Basmati showing above 85% genetic similarity with the same. Further selection was continued till F5 generation for higher recovery for Type 3 Basmati characteristics. The desirable alleles of intermediate amylose content (wx) and aroma (fgr) loci of Type 3 Basmati were also tracked using the linked SSR markers. The BC2F5 pyramid lines T3-4, T3-5, T3-6 and T3-7 homozygous for the three target genes Xa21, xa13 and sd-1 from the donor parent with wx and fgr alleles of Type 3 Basmati had excellent cooking quality and strong aroma. Springer Science+Business Media B.V., 2010 Basmati rice nationallicence Bacterial blight nationallicence Resistance gene nationallicence Xanthomonas oryzae pv. oryzae nationallicence MAS nationallicence Background selection nationallicence Pyramiding nationallicence Semidwarf nationallicence Rajpurohit Deepak Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Kumar Rahul Department of Biotechnology, ICFAI University, Dehradun, UA, India aut Kumar Mankesh Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Paul Priyanka Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Awasthi Anjali Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Osman Basha P. Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Puri Anju Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Jhang Tripta Central Institute of Medicinal and Aromatic Plants, CSIR, Lucknow, UP, India aut Singh Kuldeep School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India aut Dhaliwal Harcharan Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut Euphytica Springer Netherlands 178/1(2011-03-01), 111-126 0014-2336 178:1<111 2011 178 10681 https://doi.org/10.1007/s10681-010-0279-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0279-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rajpurohit Deepak Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Kumar Rahul Department of Biotechnology, ICFAI University, Dehradun, UA, India aut NATIONALLICENCE 700 1- Kumar Mankesh Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Paul Priyanka Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Awasthi Anjali Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Osman Basha P. Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Puri Anju Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 700 1- Jhang Tripta Central Institute of Medicinal and Aromatic Plants, CSIR, Lucknow, UP, India aut NATIONALLICENCE 700 1- Singh Kuldeep School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India aut NATIONALLICENCE 700 1- Dhaliwal Harcharan Department of Biotechnology, Indian Institute of Technology, 247 667, Roorkee, UA, India aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/1(2011-03-01), 111-126 0014-2336 178:1<111 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer