caa a22 4500 44532385X CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10681-010-0310-0 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0310-0 Characterization of resistance gene analogs from Gossypium arboreum and their evolutionary relationships with homologs from tetraploid cottons [Elektronische Daten] [Muhammad Azhar, Imran Amin, Aftab Bashir, Shahid Mansoor] Four cotton species (genus Gossypium) produce spinable fiber. The two diploid species of Asiatic origin, Gossypium arboreum and G. herbaceum, have been largely replaced by G. hirsutum. However, these diploid species are potentially a rich source of genes for the improvement of G. hrisutum, particularly in terms of providing resistance against biotic and abiotic stresses. As a first step towards understanding the mechanisms of resistance in cotton, we designed 24 non-degenerate primers based on resistance gene analogs (RGAs) cloned from G. hirsutum for screening a number of cotton species with the A and D genomes. Most of these RGAs are conserved on the A genome (G. arboreum), suggesting a bias towards this genome. The amplified RGAs from G. arboreum were cloned and their nucleotide and amino acid sequences compared with RGA sequences available in public databases. The majority of the RGAs identified were homologous to those isolated from G. hirsutum and G. barbadense, but their diversity was greater than expected at both the nucleotide and amino acid levels. These RGAs provide useful tools for the identification of full-length resistance genes from bacterial artificial chromosome and cDNA libraries. Springer Science+Business Media B.V., 2010 Gossypium arboreum nationallicence RGAs nationallicence NBS-LRR nationallicence Azhar Muhammad Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut Amin Imran Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut Bashir Aftab Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut Mansoor Shahid Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut Euphytica Springer Netherlands 178/3(2011-04-01), 351-362 0014-2336 178:3<351 2011 178 10681 https://doi.org/10.1007/s10681-010-0310-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0310-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Azhar Muhammad Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Amin Imran Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Bashir Aftab Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Mansoor Shahid Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/3(2011-04-01), 351-362 0014-2336 178:3<351 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer