caa a22 4500 445323876 CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10681-010-0316-7 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0316-7 Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase [Elektronische Daten] [Zi-Hui Liu, Hong-Mei Zhang, Guo-Liang Li, Xiu-Lin Guo, Shou-Yi Chen, Gui-Bo Liu, Yan-Min Zhang] Gene BADH was transformed into alfalfa (Medicago sativa L.) through Agrobacterium-mediated transformation method, and salt tolerance of the transgenic progenies was assessed. Among 247 plants obtained after transformation, 43 were positive by polymerase chain reaction (PCR) detection using the primers specific for BADH gene. Reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that gene BADH was expressed in 42 plants at transcriptional level. Southern blotting analysis also showed that the target was integrated into Medicago genome. The transgenic plants grew vigorous in salt stress condition, whereas the wild type plants was retarded and did not survive after cradle. The relative electrical conductivity and malondialdehyde (MDA) contents in the T1 transgenic plants were lower, but peroxidase (POD) and superoxide dismutase (SOD) activities were higher than those of the wild type plants. These results demonstrated that the expression of foreign BADH gene enhanced the salt tolerance in transgenic alfalfa. Springer Science+Business Media B.V., 2010 Medicago sativa L nationallicence BADH nationallicence Transformation nationallicence Salt tolerance nationallicence ABA : Abscisic acid nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence SOD : Superoxide dismutase nationallicence POD : Peroxidase nationallicence MDA : Malondialdehyde nationallicence AS : Acetosyringone nationallicence Liu Zi-Hui Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut Zhang Hong-Mei Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut Li Guo-Liang Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut Guo Xiu-Lin Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut Chen Shou-Yi Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Liu Gui-Bo Institute of Dry-Land Farming, Hebei Academy of Agriculture and Forestry Sciences, 053000, Hengshui, China aut Zhang Yan-Min Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut Euphytica Springer Netherlands 178/3(2011-04-01), 363-372 0014-2336 178:3<363 2011 178 10681 https://doi.org/10.1007/s10681-010-0316-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0316-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zi-Hui Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut NATIONALLICENCE 700 1- Zhang Hong-Mei Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut NATIONALLICENCE 700 1- Li Guo-Liang Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut NATIONALLICENCE 700 1- Guo Xiu-Lin Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut NATIONALLICENCE 700 1- Chen Shou-Yi Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Liu Gui-Bo Institute of Dry-Land Farming, Hebei Academy of Agriculture and Forestry Sciences, 053000, Hengshui, China aut NATIONALLICENCE 700 1- Zhang Yan-Min Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, 050051, Shijiazhuang, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/3(2011-04-01), 363-372 0014-2336 178:3<363 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer