caa a22 4500 445323914 CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10681-010-0317-6 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0317-6 Could EST-based markers be used for the marker-assisted selection of drought tolerant barley ( Hordeum vulgare ) lines? [Elektronische Daten] [Fruzsina Szira, Andreas Börner, Kerstin Neumann, Khalil Nezhad, Gábor Galiba, András Bálint] To improve our knowledge on the genetic control of drought tolerance, the Oregon Wolfe Barleys (OWB), considered as a reference population in genetic mapping, were subjected to various types of water deficit. Overall, when investigating numerous environments and replications, 40 QTLs were identified in three developmental stages. Based on these loci five QTL clusters were separated, which affect various drought-related traits in at least two developmental stages. Several candidate genes were identified for each QTL cluster using an expressed sequence tag (EST)-based map with high marker density. The putative role of the candidates in drought tolerance is discussed. The phenotypic effect of three of the five candidate genes was also tested on 39 barley landraces and cultivars and a significant relationship was found between the allelic composition of these genes and yield production under stress conditions. This study presents a relevant example of the use of reliable QTL data in the candidate gene approach, while also demonstrating how the results could be practically utilized in marker-assisted selection (MAS). Springer Science+Business Media B.V., 2010 Candidate genes nationallicence Developmental stage nationallicence Drought nationallicence Marker-assisted selection nationallicence Osmotic stress nationallicence QTL nationallicence Szira Fruzsina Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut Börner Andreas Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut Neumann Kerstin Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut Nezhad Khalil Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut Galiba Gábor Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut Bálint András Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut Euphytica Springer Netherlands 178/3(2011-04-01), 373-391 0014-2336 178:3<373 2011 178 10681 https://doi.org/10.1007/s10681-010-0317-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0317-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szira Fruzsina Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut NATIONALLICENCE 700 1- Börner Andreas Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Neumann Kerstin Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Nezhad Khalil Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Galiba Gábor Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut NATIONALLICENCE 700 1- Bálint András Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, 2462, Martonvásár, Hungary aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/3(2011-04-01), 373-391 0014-2336 178:3<373 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer