caa a22 4500 445323922 CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10681-010-0319-4 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0319-4 Investigation of pollen competition between wild and cultivated sorghums ( Sorghum bicolor (L.) Moench) using simple sequence repeats markers [Elektronische Daten] [Moses Muraya, Hartwig Geiger, Santie de Villiers, Fabrice Sagnard, Ben Kanyenji, Dan Kiambi, Heiko Parzies] In self-compatible plant species stigmata receive a mixture of self and outcrossed pollen and competition between them is expected to play a major role in determining the pollen-mediated gene flow. The use of male sterile bait plants in field trials to demonstrate the rate of gene flow is questionable due to lack of pollination competition. However, little direct evidence has been published. A field experiment of male sterile and male fertile sorghum pollen recipient bait plants was conducted to evaluate pollen competition between wild and cultivated sorghums and the effects of pollen competition on gene flow assessment. Pollen competition between wild and cultivated sorghums was estimated from two-component pollen mixtures of wild and cultivated sorghum (1:1 ratio) applied to wild, cultivated and male-sterile maternal bait plants. Paternity was determined in the progeny using two diagnostic Simple Sequence Repeat markers. The study found that self pollen has higher seed-siring success. Maternal genotype influences the siring ability of the pollen donor components which significantly deviated from the 1:1 pollen loads. The study showed that published estimates of gene flow derived from studies using male-sterile bait plants seriously overestimate gene flow and that pollen competition may be a significant factor influencing outcrossing rates. The results suggest that the predominant direction of gene flow is from cultivated to wild sorghum, potentially leading to introgression of crop genes into wild sorghum. Pollen competition should be taken into account in gene flow estimation, since presence of self-pollen can account for over half of seed produced irrespective of maternal genotype. Springer Science+Business Media B.V., 2010 Sorghum bicolor nationallicence Pollen competition nationallicence Outcross pollen nationallicence Self pollen nationallicence Pollination nationallicence Pollen-mediated gene flow nationallicence Muraya Moses Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut Geiger Hartwig Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Fruwirthstrasse 21, 70599, Stuttgart, Germany aut de Villiers Santie International Crops Research Institute for the Semi-Arid Tropics (ICRISAT-Nairobi), P.O. Box 39063-00623, Nairobi, Kenya aut Sagnard Fabrice CIRAD—UMR Développement et Amélioration des Plantes, c/o ILRI, P.O. Box 30709, Nairobi, Kenya aut Kanyenji Ben Kenya Agricultural Research Institute (KARI-Embu), P.O. Box 30148, Nairobi, Kenya aut Kiambi Dan International Crops Research Institute for the Semi-Arid Tropics (ICRISAT-Nairobi), P.O. Box 39063-00623, Nairobi, Kenya aut Parzies Heiko Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Fruwirthstrasse 21, 70599, Stuttgart, Germany aut Euphytica Springer Netherlands 178/3(2011-04-01), 393-401 0014-2336 178:3<393 2011 178 10681 https://doi.org/10.1007/s10681-010-0319-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0319-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muraya Moses Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Geiger Hartwig Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Fruwirthstrasse 21, 70599, Stuttgart, Germany aut NATIONALLICENCE 700 1- de Villiers Santie International Crops Research Institute for the Semi-Arid Tropics (ICRISAT-Nairobi), P.O. Box 39063-00623, Nairobi, Kenya aut NATIONALLICENCE 700 1- Sagnard Fabrice CIRAD—UMR Développement et Amélioration des Plantes, c/o ILRI, P.O. Box 30709, Nairobi, Kenya aut NATIONALLICENCE 700 1- Kanyenji Ben Kenya Agricultural Research Institute (KARI-Embu), P.O. Box 30148, Nairobi, Kenya aut NATIONALLICENCE 700 1- Kiambi Dan International Crops Research Institute for the Semi-Arid Tropics (ICRISAT-Nairobi), P.O. Box 39063-00623, Nairobi, Kenya aut NATIONALLICENCE 700 1- Parzies Heiko Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Fruwirthstrasse 21, 70599, Stuttgart, Germany aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/3(2011-04-01), 393-401 0014-2336 178:3<393 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer