caa a22 4500 445323930 CHVBK 20180317142713.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10681-010-0343-4 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0343-4 Evaluation of seedling cold tolerance in rice cultivars: a comparison of visual ratings and quantitative indicators of physiological changes [Elektronische Daten] [Sang-Ic Kim, Thomas Tai] Rice (Oryza sativa L.) is sensitive to prolonged exposure to low temperature, which at the seedling stage can result in significant chilling injury and mortality. The objective of this study was to quantify physiological and biochemical changes in rice seedlings undergoing chilling stress and compare those changes with visual evaluation of tolerance. Seedlings from the cultivars M-202 (tolerant) and IR50 (sensitive) were subjected to 9°C for 14days in a controlled environment chamber. Leaf tissues were harvested at various time points for determination of electrolyte leakage, proline, malondialdehyde, ascorbic acid and reduced glutathione. Significant differences between M-202 and IR50 were detected in electrolyte leakage, proline, and ascorbic acid starting at 7days with IR50 exhibiting higher levels of these indicators. Most IR50 seedlings were dead at 14days. A set of fifty rice accessions including M-202 and IR50 was evaluated at 10days of cold treatment to examine the correlation of visual ratings with the physiological indices. Visual ratings were most highly correlated with electrolyte leakage and least correlated with proline content. Based on visual ratings and the physiological indices, we identified several cultivars that outperformed M-202 in cold tolerance while IR50 had the lowest tolerance of the cultivars tested. Springer Science+Business Media B.V. (outside the USA), 2011 Oryza sativa nationallicence Rice nationallicence Seedling nationallicence Cold tolerance nationallicence Electrolyte leakage nationallicence Antioxidants nationallicence Lipid peroxidation nationallicence Proline nationallicence Kim Sang-Ic USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences—Mail Stop 1, University of California, 95616, Davis, CA, USA aut Tai Thomas USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences—Mail Stop 1, University of California, 95616, Davis, CA, USA aut Euphytica Springer Netherlands 178/3(2011-04-01), 437-447 0014-2336 178:3<437 2011 178 10681 https://doi.org/10.1007/s10681-010-0343-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0343-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Sang-Ic USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences—Mail Stop 1, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Tai Thomas USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences—Mail Stop 1, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/3(2011-04-01), 437-447 0014-2336 178:3<437 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer