caa a22 4500 445323981 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10681-010-0297-6 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0297-6 Overcoming pre-fertilization barriers in the wide cross between Chrysanthemum grandiflorum (Ramat.) Kitamura and C. nankingense (Nakai) Tzvel. by using special pollination techniques [Elektronische Daten] [Chun-Qing Sun, Zhi-Zhe Huang, Yan-Li Wang, Fa-Di Chen, Nian-Jun Teng, Wei-Min Fang, Zhao-Lei Liu] In our earlier study, pre-fertilization barrier was one of the main factors causing the failure of the wide hybridization between Chrysanthemum grandiflorum ‘Yuhuaxingchen' and C. nankingense, and seriously restricted the utilization of the excellent chrysanthemum germplasm, C. nankingense. In order to overcome the pre-fertilization barrier and obtain some hybrids, we carried out wide hybridization between them again in this study, and tentatively adopted three pollination techniques including mentor pollen, delayed pollination, and gibberellic acid treatment. The average number of pollen grains germinating on stigma at 24h after pollination and the percentage of embryo were investigated with a temporary mount method and the technique of fluorescence microscopy. It was found that, compared with the control, delayed pollination, gibberellic acid treatment, and mentor pollen significantly increased the average number of pollen grains germinating on each stigma at 24h after pollination by approximately 152, 235, and 182%, respectively. In addition, all the three pollination techniques significantly increased the percentage of normal embryos at different periods after artificial pollination in comparison to the control. Furthermore, seed sets of the crosses using the three special pollination techniques were 1.1, 1.4, and 2.0%, respectively, whereas we did not obtained any seeds in the control. Taken together, these results suggest that delayed pollination, gibberellic acid treatment, and mentor pollen are three useful pollination techniques for overcoming pre-fertilization barriers and increasing seed set in the wide hybridization between C. grandiflorum ‘Yuhuaxingchen' and C. nankingense, and they may be widely applied for increasing breeding efficiency in other chrysanthemum wide hybridization facing similar pre-fertilization barriers. Springer Science+Business Media B.V., 2010 Chrysanthemum grandiflorum nationallicence Delayed pollination nationallicence Gibberellic acid nationallicence Mentor pollen nationallicence Pre-fertilization barrier nationallicence Wide cross nationallicence Sun Chun-Qing College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Huang Zhi-Zhe College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Wang Yan-Li College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Chen Fa-Di College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Teng Nian-Jun College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Fang Wei-Min College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Liu Zhao-Lei College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut Euphytica Springer Netherlands 178/2(2011-03-01), 195-202 0014-2336 178:2<195 2011 178 10681 https://doi.org/10.1007/s10681-010-0297-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0297-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Chun-Qing College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Huang Zhi-Zhe College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Wang Yan-Li College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Chen Fa-Di College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Teng Nian-Jun College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Fang Wei-Min College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Liu Zhao-Lei College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/2(2011-03-01), 195-202 0014-2336 178:2<195 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer