caa a22 4500 445324015 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10681-010-0293-x doi (NATIONALLICENCE)springer-10.1007/s10681-010-0293-x Mating systems in tropical forages: Stylosanthes capitata Vog. and Stylosanthes guianensis (Aubl.) Sw [Elektronische Daten] [Melissa de Santos-Garcia, Rosângela Resende, Lucimara Chiari, Maria Zucchi, Anete de Souza] Stylosanthes capitata and S. guianensis are important forage legumes for tropical areas. The only available estimates of S. capitata and S. guianensis outcrossing rates were based on morphological markers, and the genus is considered as being mainly self-pollinated. Here we describe an estimation of the outcrossing rate in S. capitata and S. guianensis using microsatellite markers. The outcrossing rates were estimated in S. capitata and S. guianensis open-pollinated populations of 20 progenies consisting of ten individuals each. The multi locus outcrossing rate for S. capitata was estimated using 10 polymorphic loci, whereas five microsatellites were used for S. guianensis. The multi locus outcrossing rates for S. capitata and S. guianensis were 31 and 26%, respectively, suggesting a mixed mating system with predominance of autogamy. Comparison of single locus and multi locus estimates of outcrossing rates indicated that little inbreeding other than selfing occurred. The estimated Wright's fixation index of the parental generation was lower than expected based on the multi locus outcrossing rate, possibly resulting from the use of some heterozygous breeding genotypes for the study. The data on the outcrossing rate described here are potentially useful for breeding programs and for maintenance of germplasm collections of these Stylosanthes species. Springer Science+Business Media B.V., 2010 Microsatellite markers nationallicence Autogamy nationallicence Mixed mating system nationallicence Plant breeding nationallicence Santos-Garcia Melissa de Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Barão Geraldo, CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Resende Rosângela Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Gado de Corte. BR 262, Km 4, CEP 79002-970, Campo Grande, MS, Brazil aut Chiari Lucimara Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Gado de Corte. BR 262, Km 4, CEP 79002-970, Campo Grande, MS, Brazil aut Zucchi Maria Pólo Apta Centro Sul, Rod. SP 127km 30, CP 28, CEP 13400-970, Piracicaba, SP, Brazil aut de Souza Anete Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Barão Geraldo, CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Euphytica Springer Netherlands 178/2(2011-03-01), 185-193 0014-2336 178:2<185 2011 178 10681 https://doi.org/10.1007/s10681-010-0293-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0293-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santos-Garcia Melissa de Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Barão Geraldo, CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Resende Rosângela Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Gado de Corte. BR 262, Km 4, CEP 79002-970, Campo Grande, MS, Brazil aut NATIONALLICENCE 700 1- Chiari Lucimara Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Gado de Corte. BR 262, Km 4, CEP 79002-970, Campo Grande, MS, Brazil aut NATIONALLICENCE 700 1- Zucchi Maria Pólo Apta Centro Sul, Rod. SP 127km 30, CP 28, CEP 13400-970, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- de Souza Anete Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Barão Geraldo, CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 178/2(2011-03-01), 185-193 0014-2336 178:2<185 2011 178 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer