caa a22 4500 445324066 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0430-1 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0430-1 Confirmation of quantitative trait loci for resistance to multiple-HG types of soybean cyst nematode ( Heterodera glycines Ichinohe) [Elektronische Daten] [T. Vuong, D. Sleper, J. Shannon, X. Wu, H. Nguyen] Genetic analysis of resistance of plant introduction (PI) 438489B to soybean cyst nematode (SCN) have shown that this PI is highly resistant to many SCN HG types. However, validation of the previously detected quantitative trait loci (QTL) has not been done. In this study, 250 F2:3 progeny of a Magellan (susceptible)×PI 438489B (resistant) cross were used for primary genetic mapping to detect putative QTL for resistance to five SCN HG types. QTL confirmation study was subsequently conducted using F6:7 recombinant inbred lines (RILs) derived from the same cross. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were employed for molecular genotyping. Interval mapping (IM), permutation tests, cofactor selection, and composite interval mapping (CIM) were performed to identify and map QTL. Results showed that five QTL intervals were associated with resistance to either multiple- or single-HG types of SCN. Among these, two major QTL for resistance to multiple-SCN HG types were mapped to chromosomes (Chr.) 8 and 18, consistent with the known rhg1 and Rhg4 locations. The other QTL were mapped to Chr. 4. The results of our study confirmed earlier reported SCN resistance QTL in this PI. Moreover, SSR and SNP molecular markers tightly linked to these QTL can be useful for the near-isogenic lines (NILs) development aiming to fine-mapping of these QTL regions and map-based cloning of SCN resistance candidate genes. Springer Science+Business Media B.V., 2011 Soybean ( Glycine max ) nationallicence Soybean cyst nematode nationallicence Quantitative trait loci nationallicence Simple sequence repeat nationallicence Single nucleotide polymorphisms nationallicence Vuong T. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut Sleper D. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut Shannon J. Division of Plant Sciences and NCSB, University of Missouri-Columbia, Delta Center, P.O. Box 160, 63873, Portageville, MO, USA aut Wu X. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut Nguyen H. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut Euphytica Springer Netherlands 181/1(2011-09-01), 101-113 0014-2336 181:1<101 2011 181 10681 https://doi.org/10.1007/s10681-011-0430-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0430-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vuong T. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Sleper D. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Shannon J. Division of Plant Sciences and NCSB, University of Missouri-Columbia, Delta Center, P.O. Box 160, 63873, Portageville, MO, USA aut NATIONALLICENCE 700 1- Wu X. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Nguyen H. Division of Plant Sciences and National Center for Soybean Biotechnology (NCSB), University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 101-113 0014-2336 181:1<101 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer