caa a22 4500 445324074 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0421-2 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0421-2 Mapping resistance gene analogs (RGAs) in cultivated tetraploid cotton using RGA-AFLP analysis [Elektronische Daten] [Chen Niu, Yingzhi Lu, Youlu Yuan, Richard Percy, Mauricio Ulloa, Jinfa Zhang] Diseases cause significant losses in cotton production throughout the US Cotton Belt. Growing resistant cultivars can significantly improve cotton yields and effectively reduce production inputs. Disease resistance (R) genes have been isolated in numerous plant species and the R genes with domains of nucleotide binding sites (NB) and leucine rich repeats (LRR) represent the largest R gene family. Degenerate primers designed based on conserved motifs of plant disease resistance genes were used alone or in combination with AFLP primers to analyze disease resistance gene analogs (RGAs) in a recombinant inbred line (RIL) population of Pima (Gossypium barbadense) 3-79 and Upland cotton (G. hirsutum) line NM 24016. Eighty-eight polymorphic RGA markers were amplified by 8 pairs of RGA degenerate primers, while 131 polymorphic RGA-AFLP markers were produced from six pairs of RGA-AFLP primer combinations. Of the 219 polymorphic RGA and RGA-AFLP markers that were identified, 212 were assigned to 18 chromosomes and linkage groups based on existing SSR markers that are on known chromosomes. However, the RGA and RGA-AFLP markers are not evenly distributed among chromosomes in that 189 RGA and RGA-AFLP markers (88%) are assigned onto three "giant” chromosomes, i.e., C6, C12, and C15, suggesting RGA clusters in the cotton genome. Several RGA and RGA-AFLP markers were mapped to the same linkage group carrying a root-knot nematode resistance gene. The identification and mapping of RGA and RGA-AFLP markers provide a framework to facilitate marker-assisted selection of disease resistance in cotton breeding and to understand the physical relationship of cotton resistance genes. Springer Science+Business Media B.V., 2011 Cotton nationallicence Linkage map nationallicence Recombinant inbred line (RIL) nationallicence Resistance gene analogs (RGAs) nationallicence RGA-AFLP nationallicence Niu Chen Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut Lu Yingzhi Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut Yuan Youlu Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut Percy Richard Crop Germplasm Research, USDA-ARS, 2881 F&B ROAD, 77845, College Station, TX, USA aut Ulloa Mauricio USDA-ARS, WICS, Res. Unit, Cotton Enhancement Program, 93263, Shafter, CA, USA aut Zhang Jinfa Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut Euphytica Springer Netherlands 181/1(2011-09-01), 65-76 0014-2336 181:1<65 2011 181 10681 https://doi.org/10.1007/s10681-011-0421-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0421-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niu Chen Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 700 1- Lu Yingzhi Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 700 1- Yuan Youlu Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 700 1- Percy Richard Crop Germplasm Research, USDA-ARS, 2881 F&B ROAD, 77845, College Station, TX, USA aut NATIONALLICENCE 700 1- Ulloa Mauricio USDA-ARS, WICS, Res. Unit, Cotton Enhancement Program, 93263, Shafter, CA, USA aut NATIONALLICENCE 700 1- Zhang Jinfa Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 65-76 0014-2336 181:1<65 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer