caa a22 4500 445324082 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0370-9 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0370-9 Agrobacterium -mediated transformation of cotton ( Gossypium hirsutum L.) with a fungal phytase gene improves phosphorus acquisition [Elektronische Daten] [J. Liu, C. Zhao, J. Ma, G. Zhang, M. Li, G. Yan, X. Wang, Z. Ma] A phytase gene (phyA), isolated from Aspergillus ficuum (AF537344), was introduced into cotton (Gossypium hirsutum L.) by Agrobacterium-mediated transformation to increase the phosphorus (P) acquisition efficiency of cotton. Southern and Northern blot analyses showed that the phyA was successfully incorporated into the cotton genome and expressed in transgenic lines. After growing for 45days with phytate (Po) as the only P source, the shoot and root dry weights of the transgenic plants all increased by nearly 2.0-fold relative to those of wild-type plants, but were similar to those of transgenic plants supplied with inorganic phosphorus. The phytase activities of root extracts prepared from transgenic plants were 2.4- to 3.6-fold higher than those from wild-type plants, and the extracellular phytase activities of transgenic plants were also 4.2- to 6.3-fold higher. Furthermore, the expressed phytase was secreted into the rhizospheres as demonstrated by enzyme activity staining. The transgenic plants accumulated much higher contents of total P (up to 2.1-fold after 30days of growth) than the wild-type plants when supplied with Po. These findings clearly showed that cotton plant transformed with a fungal phytase gene was able to secret the enzyme from the root, which markedly improved the plant's ability to utilize P from phytate. This may serve as a promising step toward the development of new cotton cultivars with improved phosphorus acquisition. Springer Science+Business Media B.V., 2011 Cotton ( Gossypium hirsutum L.) nationallicence Phosphorus nationallicence Phytase nationallicence Transgenic plants nationallicence Liu J. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Zhao C. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Ma J. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Zhang G. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Li M. Institute for Molecular Biology, Nankai University, 300071, Tianjin, China aut Yan G. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Wang X. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Ma Z. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut Euphytica Springer Netherlands 181/1(2011-09-01), 31-40 0014-2336 181:1<31 2011 181 10681 https://doi.org/10.1007/s10681-011-0370-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0370-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu J. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Zhao C. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Ma J. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Zhang G. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Li M. Institute for Molecular Biology, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Yan G. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Wang X. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 700 1- Ma Z. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, 071001, Baoding, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 31-40 0014-2336 181:1<31 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer