caa a22 4500 445324090 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0380-7 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0380-7 Inheritance of somatic embryogenesis using leaf petioles as explants in upland cotton [Elektronische Daten] [Chaojun Zhang, Shuxun Yu, Shuli Fan, Jinfa Zhang, Fuguang Li] Somatic embryogenesis (SE) is a critical step leading to plant regeneration in tissue culture of many plant species. The objective of the present study was to analyze the inheritance of SE in cotton (Gossypium hirsutum L.) using leaf petioles as explants. A high embryogenic callus (HEC)—producing line, W10, was selected by petiole callus culture from a commercial Chinese cotton cultivar CRI24 and crossed with a non embryogenic line, TM-1 and a low embryogenic (LEC) commercial Chinese cotton cultivar, CRI12, respectively. The parental lines, F1 and F2 were grown in field conditions for sources of leaf petioles as explants. The F1 plants were similar to the HEC parent in embryogenic callus (EC) induction, indicating that high EC ability is dominant. The classical Mendelian analysis showed that the high EC ability in the HEC line W10 is controlled by two independent dominant genes with complementary effect, designated Ec 1 and Ec 2, while the LEC line CRI12 contains one dominant gene Ec 2. A joint segregation analysis confirmed that SE ability in cotton is controlled by two major genes with epistatic effects along with other polygenes. A SSR marker analysis identified three quantitative trait loci (QTLs) on two linkage groups, one of which harbored a major QTL (qEc1) which is assigned to the major gene Ec 1. This qualitative and quantitative genetic study has provided an incentive to fine map the genes responsible for SE towards the isolation of the SE genes in cotton. Springer Science+Business Media B.V., 2011 Cotton nationallicence Somatic embryogenesis nationallicence Inheritance nationallicence SSR markers nationallicence Zhang Chaojun China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut Yu Shuxun China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut Fan Shuli China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut Zhang Jinfa Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut Li Fuguang China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut Euphytica Springer Netherlands 181/1(2011-09-01), 55-63 0014-2336 181:1<55 2011 181 10681 https://doi.org/10.1007/s10681-011-0380-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0380-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Chaojun China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut NATIONALLICENCE 700 1- Yu Shuxun China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut NATIONALLICENCE 700 1- Fan Shuli China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jinfa Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 700 1- Li Fuguang China Cotton Research Institute, 455112, Anyang, Henan, People's Republic of China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 55-63 0014-2336 181:1<55 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer