caa a22 4500 445324147 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0354-9 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0354-9 Improved salt tolerance and seed cotton yield in cotton ( Gossypium hirsutum L.) by transformation with betA gene for glycinebetaine synthesis [Elektronische Daten] [Kewei Zhang, Ning Guo, Lijun Lian, Juan Wang, Sulian Lv, Juren Zhang] Homozygous transgenic cotton (Gossypium hirsutum L.) plants that accumulated glycinebetaine (GB) in larger quantities were more tolerant to salt than wild-type (WT) plants. Four transgenic lines, namely 1, 3, 4, and 5, accumulated significantly higher levels of GB than WT plants did both before and after salt stress. At 175 and 275mM NaCl, seeds of all the transgenic lines germinated earlier and recorded a higher final germination percentage, and the seedlings grew better, than those of the WT. Under salt stress, all the lines showed some characteristic features of salt tolerance, such as higher leaf relative water content (RWC), higher photosynthesis, better osmotic adjustment (OA), lower percentage of ion leakage, and lower peroxidation of the lipid membrane. Levels of endogenous GB in the transgenic plants were positively correlated with RWC and OA. The results indicate that GB in transgenic cotton plants not only maintains the integrity of cell membranes but also alleviates osmotic stress caused by high salinity. Lastly, the seed cotton yield of transgenic lines 4 and 5 was significantly higher than that of WT plants in saline soil. This research indicates that betA gene has the potential to improve crop's salt tolerance in areas where salinity is limiting factors for agricultural productivity. Springer Science+Business Media B.V., 2011 Salt stress nationallicence Glycinebetaine nationallicence Transgenic cotton nationallicence Seed cotton yield nationallicence BADH : Betaine aldehyde dehydrogenase nationallicence CDH : Choline dehydrogenase nationallicence CMO : Choline monooxygenase nationallicence GB : Glycinebetaine nationallicence MDA : Malondialdehyde nationallicence OA : Osmotic adjustment nationallicence ROS : Reactive oxygen species nationallicence RWC : Relative water content nationallicence SOD : Superoxide dismutase nationallicence WT : Wild-type nationallicence Zhang Kewei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Guo Ning School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Lian Lijun School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Wang Juan School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Lv Sulian School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Zhang Juren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut Euphytica Springer Netherlands 181/1(2011-09-01), 1-16 0014-2336 181:1<1 2011 181 10681 https://doi.org/10.1007/s10681-011-0354-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0354-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Kewei School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Guo Ning School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Lian Lijun School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Wang Juan School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Lv Sulian School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Zhang Juren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, Shandong, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 1-16 0014-2336 181:1<1 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer