caa a22 4500 445324163 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0422-1 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0422-1 Microsporogenesis of Rps 8/ rps 8 heterozygous soybean lines [Elektronische Daten] [Maria Ortega, Anne Dorrance] Phytophthora root and stem rot caused by Phytophthora sojae, is one of the most damaging diseases of soybean, for which management is principally done by planting resistant cultivars with race specific resistance which are conferred by Rps (Resistance to Phytophthora sojae) genes. The Rps8 locus, identified in the South Korean landrace PI 399073, is located in a 2.23Mbp region on soybean chromosome 13. In eight cv. Williams (rps8/rps8)×PI 399073 (Rps8/Rps8) populations, this region exhibited strong segregation distortion. In a cross between the South Korean lines PI 399073 (Rps8/Rps8) and PI 408211B (multiple Rps genes) this region segregated in a Mendelian fashion. In this study, microsporogenesis was evaluated to identify meiotic abnormalities that may be associated with the segregation distortion of the Rps8 region. Pollen was collected from greenhouse-grown plants of the parental genotypes: Williams, PI 399073, and PI 408211B; as well as selected Rps8/rps8 RILs from Williams×PI 399073 BC4F2:3 and PI 399073×PI 408211B F4:5 populations. There were no differences for pollen viability among the genotypes. However, for PI 399073, a mix of dyads, triads, tetrads and pentads was observed. A high frequency of meiotic abnormalities including fragments, laggards, multinucleated microspores; and microcytes containing DNA was also observed in Rps8/rps8 Williams×PI 399073 BC4F2:3 RILs. These meiotic abnormalities may contribute to the high degree of segregation distortion present in the Williams×PI 399073 populations. The Author(s), 2011 Microsporogenesis nationallicence Segregation distortion nationallicence Soybean nationallicence Pollen nationallicence Resistance gene nationallicence Ortega Maria Department of Plant Pathology, The Ohio State University, 44691, Wooster, OH, USA aut Dorrance Anne Department of Plant Pathology, The Ohio State University, 44691, Wooster, OH, USA aut Euphytica Springer Netherlands 181/1(2011-09-01), 77-88 0014-2336 181:1<77 2011 181 10681 https://doi.org/10.1007/s10681-011-0422-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0422-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortega Maria Department of Plant Pathology, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Dorrance Anne Department of Plant Pathology, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/1(2011-09-01), 77-88 0014-2336 181:1<77 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer