caa a22 4500 44532421X CHVBK 20180317142714.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10681-011-0428-8 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0428-8 Development of gene-based markers from functional Arabidopsis thaliana genes involved in phosphorus homeostasis and mapping in Brassica napus [Elektronische Daten] [Guangda Ding, Yuan Liao, Mei Yang, Zunkang Zhao, Lei Shi, Fangsen Xu] Brassica napus is an important oilseed and fodder crop grown throughout the world. Although it is widely grown, little is known about the molecular basis of phosphorus (P) homeostasis for this species. In this research, a population of 124 recombinant inbred lines (RILs) (designated as BE-RIL) derived from a cross between P-inefficient cv. ‘B104-2' and P-efficient cv. ‘Eyou Changjia' was used to construct a genetic map of P homeostasis genes. A set of gene-based markers (GBMs) was developed from functional genes involved in Arabidopsis thaliana P homeostasis. In total, 46 GBMs corresponding to 26 genes, assigned to eight functional categories, were integrated into the BE-RIL map. A total of 243 simple sequence repeat (SSR) markers were developed from 171 bacterial artificial chromosome (BAC) end sequences and/or B. rapa seed BAC sequences. Of these SSR markers, 74 were added to the BE-RIL map. Based on the newly constructed genetic map, comparative genetic analysis between A. thaliana and B. napus was performed. A total of 90 conserved genomic blocks were aligned between A. thaliana pseudochromosomes and the BE-RIL linkage groups. According to physical positions on the Arabidopsis genome, 1223 orthologs of 356 genes involved in Arabidopsis P homeostasis were mapped onto syntenic blocks and insertion segments. This high-density genetic map will be useful for identifying quantitative trait loci (QTL) that control P homeostasis and putative candidate genes for the efficient use of P in B. napus. Springer Science+Business Media B.V., 2011 Brassica napus nationallicence P homeostasis nationallicence RIL population nationallicence Gene-based marker nationallicence SSR nationallicence Comparative genetic analysis nationallicence P : Phosphorus nationallicence RIL : Recombinant inbred line nationallicence GBM : Gene-based marker nationallicence SSR : Single sequence repeat nationallicence QTL : Quantitative trait loci nationallicence Ding Guangda National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Liao Yuan National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Yang Mei National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Zhao Zunkang National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Shi Lei National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Xu Fangsen National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Euphytica Springer Netherlands 181/3(2011-10-01), 305-322 0014-2336 181:3<305 2011 181 10681 https://doi.org/10.1007/s10681-011-0428-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0428-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ding Guangda National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Liao Yuan National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Yang Mei National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhao Zunkang National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Shi Lei National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Xu Fangsen National Key Laboratory of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/3(2011-10-01), 305-322 0014-2336 181:3<305 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer