caa a22 4500 445324236 CHVBK 20180317142714.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10681-011-0445-7 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0445-7 Allele mining and haplotype discovery in barley candidate genes for drought tolerance [Elektronische Daten] [András Cseri, Mátyás Cserháti, Maria von Korff, Bettina Nagy, Gábor Horváth, András Palágyi, János Pauk, Dénes Dudits, Ottó Törjék] In the present study, allele mining was conducted on a panel of drought related candidate genes in a set of 96 barley genotypes using EcoTILLING, which is a variant of the targeting induced local lesions in genomes (TILLING) technology. Analyzing approximately 1.5 million basepairs in barley a total number of 94 verified unique haplotypes were identified in 18 amplicons designed for 9 genes. Overall, 185 single nucleotide polymorphisms (SNPs) and 46 insertions/deletions (INDELs) were detected with a mean of 1SNP/92bp and 1INDEL/372bp genomic sequence. Based on overlapping haplotype sequences, markers were developed for four candidate genes (HvARH1, HvSRG6, HvDRF1, HVA1), which allows distinguishing between the main haplotypes showing either differences in amino acid sequence or which have larger INDELs in the promoter region. As "proof of concept”, the HvARH1 and HvSRG6 haplotypes were tested for the level of abscisic acid-induced gene expression in subsets of genotypes belonging to different haplotype categories. An integrated database was developed to contain information about the genes, genotypes, and haplotypes analyzed in this study. The database supplies profound information about the natural variation in the tested drought related candidate genes providing a significant asset for further mapping studies dealing with this highly polygenic trait. Springer Science+Business Media B.V., 2011 Allele mining nationallicence EcoTILLING nationallicence Drought tolerance nationallicence Candidate genes nationallicence Barley nationallicence Cseri András Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut Cserháti Mátyás Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut von Korff Maria Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Köln, Germany aut Nagy Bettina Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut Horváth Gábor Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut Palágyi András Cereal Research Non-Profit Company, Alsó kikötő sor 9, 6726, Szeged, Hungary aut Pauk János Cereal Research Non-Profit Company, Alsó kikötő sor 9, 6726, Szeged, Hungary aut Dudits Dénes Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut Törjék Ottó Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut Euphytica Springer Netherlands 181/3(2011-10-01), 341-356 0014-2336 181:3<341 2011 181 10681 https://doi.org/10.1007/s10681-011-0445-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0445-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cseri András Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Cserháti Mátyás Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- von Korff Maria Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Köln, Germany aut NATIONALLICENCE 700 1- Nagy Bettina Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Horváth Gábor Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Palágyi András Cereal Research Non-Profit Company, Alsó kikötő sor 9, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Pauk János Cereal Research Non-Profit Company, Alsó kikötő sor 9, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Dudits Dénes Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Törjék Ottó Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/3(2011-10-01), 341-356 0014-2336 181:3<341 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer