caa a22 4500 445324309 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-010-0344-3 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0344-3 Induction of Anthurium andraeanum "Arizona” tetraploid by colchicine invitro [Elektronische Daten] [Chao Chen, Xilin Hou, Hongxin Zhang, Guilan Wang, Limin Tian] Tetraploids plants of Anthurium andraeanum "Arizona” were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7h, and then were transferred into Murashige and Skoog medium containing 3mg/l BAP+0.2mg/l 2,4-D. After 60days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation. Springer Science+Business Media B.V., 2011 Anthurium andraeanum nationallicence Tetraploid nationallicence Colchicine nationallicence Flow cytometry nationallicence Variation nationallicence BAP : 6-Benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence NAA : 1-Naphtaleneacetic acid nationallicence MS : Murashige and Skoog (1962) nationallicence PI : Propidium iodide nationallicence WPB : Woody plant buffer nationallicence Chen Chao State Key Laboratory of Crop and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China aut Hou Xilin State Key Laboratory of Crop and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China aut Zhang Hongxin Department of Life Science, Tangshan Normal College, Tangshan, China aut Wang Guilan Department of Life Science, Tangshan Normal College, Tangshan, China aut Tian Limin Department of Life Science, Tangshan Normal College, Tangshan, China aut Euphytica Springer Netherlands 181/2(2011-09-01), 137-145 0014-2336 181:2<137 2011 181 10681 https://doi.org/10.1007/s10681-010-0344-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0344-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Chao State Key Laboratory of Crop and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China aut NATIONALLICENCE 700 1- Hou Xilin State Key Laboratory of Crop and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Hongxin Department of Life Science, Tangshan Normal College, Tangshan, China aut NATIONALLICENCE 700 1- Wang Guilan Department of Life Science, Tangshan Normal College, Tangshan, China aut NATIONALLICENCE 700 1- Tian Limin Department of Life Science, Tangshan Normal College, Tangshan, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 137-145 0014-2336 181:2<137 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer