caa a22 4500 445324317 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0416-z doi (NATIONALLICENCE)springer-10.1007/s10681-011-0416-z Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers [Elektronische Daten] [Ioannis Ganopoulos, Konstantinos Kazantzis, Ioannis Chatzicharisis, Irene Karayiannis, Athanasios Tsaftaris] It is important to couple phenotypic analysis with genetic diversity for germplasm conservation in gene bank collections. The use of molecular markers supports the study of genetic marker-trait associations of biological and agronomic interest on diverse genetic material. In this report, 19 Greek traditional sweet cherry cultivars and two international cultivars, which were used as controls, were grown in Greece and characterized for 17 morpho-physiological traits, 15 simple sequence repeat (SSR) loci and 10 inter simple sequence repeat (ISSR) markers. To our knowledge, this is the first report on molecular genetic diversity studies in sweet cherry in Greece. Principal component analysis (PCA) of nine qualitative and eight quantitative morphological parameters explain over 77.33% of total variability in the first five axes. The SSR markers yielded a combined matching probability ratio (MPR) of 9.569×e−12. The 15 SSR loci produced a total of 92 alleles. Ten ISSR primers generated 91 bands, with an average of 9.1 bands per primer. Expected heterozygosity (gene diversity) values of 15 SSR loci and 10 ISSR markers averaged at 0.683 and 0.369, respectively. Based on stepwise multiple regression analysis (MRA), SSR alleles were found associated with harvest time and fruit polar diameter. Furthermore, three ISSR markers were correlated with fruit harvest and soluble solids and four ISSR markers were correlated with fruit skin color. Stepwise MRA identified six SSR alleles associated with harvest time with a high correlation (P<0.001), with linear associations with high F values. Hence, data analyzed by the use of MRA could be useful in marker-assisted breeding programs when no other genetic information is available. Springer Science+Business Media B.V., 2011 Sweet cherry nationallicence SSR/ISSR nationallicence MRA nationallicence Fruit association markers nationallicence Morpho-physiological traits nationallicence SSR : Simple sequence repeat nationallicence ISSR : Inter simple sequence repeat nationallicence AFLP : Amplified fragment length polymorphism nationallicence RAPD : Random amplified polymorphic DNA nationallicence MRA : Multiple regression analysis nationallicence MPR : Matching probability ratio nationallicence PCA : Principal component analysis nationallicence PCR : Polymerase chain reaction nationallicence QTL : Quantitative trait locus nationallicence Ganopoulos Ioannis Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, 540 06, Thessaloniki, Greece aut Kazantzis Konstantinos Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut Chatzicharisis Ioannis Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut Karayiannis Irene Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut Tsaftaris Athanasios Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, 540 06, Thessaloniki, Greece aut Euphytica Springer Netherlands 181/2(2011-09-01), 237-251 0014-2336 181:2<237 2011 181 10681 https://doi.org/10.1007/s10681-011-0416-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0416-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ganopoulos Ioannis Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, 540 06, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Kazantzis Konstantinos Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut NATIONALLICENCE 700 1- Chatzicharisis Ioannis Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut NATIONALLICENCE 700 1- Karayiannis Irene Pomology Institute, NAGREF, R. S. Naousas 38, 592 00, Imathia, Greece aut NATIONALLICENCE 700 1- Tsaftaris Athanasios Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, 540 06, Thessaloniki, Greece aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 237-251 0014-2336 181:2<237 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer