caa a22 4500 445324325 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0415-0 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0415-0 Genetic studies in Centrosema pubescens benth, a tropical forage legume: the mating system, genetic variability and genetic relationships between Centrosema species [Elektronische Daten] [A. Sousa, M. Carvalho, A. Ramos, T. Campos, D. Sforça, M. Zucchi, L. Jank, A. Souza] In this study, we used microsatellite loci to estimate the outcrossing rate of Centrosema pubescens in open-pollinated populations of 10 progenies that each contained 20 genotypes. The multilocus outcrossing rate was 27%, which suggested a mixed mating system with a predominance of autogamy. The single locus outcrossing rate was 13%. The difference was 0.040, which indicated that only 4% of outcrossing occurs in related individuals. A paternity correlation of 14% suggests that there is a low probability of finding full sibs in the progeny. Cross-amplification of the 26 microsatellite loci available for C. pubescens was evaluated across 11 different Centrosema species. Nineteen of the 26 tested microsatellites were successfully transferable across the Centrosema species. The polymorphism information content and discriminating power evaluated had averages of 0.64 and 0.77, respectively. A total of three clusters were assembled to demonstrate the genetic relationships between Centrosema species. The transferable microsatellite loci should be useful for exploiting the genetic resources of the Centrosema species and determining the outcrossing rate, which are essential for proposing effective approaches for conservation and for establishing strategies for the selection and improvement of Centrosema spp. Springer Science+Business Media B.V., 2011 Tropical legume nationallicence Cross-amplification nationallicence Mating system nationallicence Autogamy nationallicence Sousa A. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Carvalho M. Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, CEP 73310-970, Planaltina, DF, Brazil aut Ramos A. Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, CEP 73310-970, Planaltina, DF, Brazil aut Campos T. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Sforça D. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Zucchi M. Agronomic Institute of Campinas, Pólo Apta Centro Sul, Rod. SP 127Km 30, CP 28, CEP 13400-970, Piracicaba, SP, Brazil aut Jank L. Forage Breeding Department, Brazilian Agricultural Research Corporation, EMBRAPA Beef Cattle, CP 154, CEP 79002-970, Campo Grande, MS, Brazil aut Souza A. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut Euphytica Springer Netherlands 181/2(2011-09-01), 223-235 0014-2336 181:2<223 2011 181 10681 https://doi.org/10.1007/s10681-011-0415-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0415-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sousa A. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Carvalho M. Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, CEP 73310-970, Planaltina, DF, Brazil aut NATIONALLICENCE 700 1- Ramos A. Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, CEP 73310-970, Planaltina, DF, Brazil aut NATIONALLICENCE 700 1- Campos T. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Sforça D. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Zucchi M. Agronomic Institute of Campinas, Pólo Apta Centro Sul, Rod. SP 127Km 30, CP 28, CEP 13400-970, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Jank L. Forage Breeding Department, Brazilian Agricultural Research Corporation, EMBRAPA Beef Cattle, CP 154, CEP 79002-970, Campo Grande, MS, Brazil aut NATIONALLICENCE 700 1- Souza A. Genetic Engineering and Molecular Biology Center (CBMEG), University of Campinas (UNICAMP), CP 6010, CEP 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 223-235 0014-2336 181:2<223 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer