caa a22 4500 44532435X CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0431-0 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0431-0 The self-incompatible RNase S-alleles of Brazilian apple cultivars [Elektronische Daten] [Celso de Albuquerque Jr., Frederico Denardi, Adriana de Mesquita Dantas, Rubens Nodari] Apple plants are self-incompatible because a genetic mechanism allows the female reproductive organ to recognize and reject self-pollen or pollen from genetic related individuals and allows non-self pollen to effect fertilization. Thus, there are implications to both breeding strategies and orchard management for fruit production. The purpose of this study was to identify and to characterize the S-RNase alleles of the gametophytic incompatibility among apple cultivars developed in Brazil, seeking to give support for choosing right combinations of parent in the apple breeding programs. It also sought to identify correct combinations of scion/pollinator cultivars of commercial apple orchards. A total of 16 specific S-RNase alleles primers were tested against DNA extracted from 12 Brazilian cultivars and their parents. The molecular analysis confronted to the reference cultivars, showed that the cultivars Daiane, Imperatriz and Princesa have the same incompatibility S3 and S5 alleles, while ‘Lisgala' showed the alleles S2 and S5; ‘Suprema', S1 and S9; ‘Catarina', S1 and S19; ‘Joaquina' and ‘Fred Hough', S5 and S19; ‘Baronesa', S3 and S9; ‘Duquesa', S2 and S3. For ‘Primícia' and ‘Condessa' it was only possible to identify one of the S-alleles, namely S24 and S2, respectively, with the second allele remaining to be identified. Progeny test indicated the Mendelian inheritance for RNase alleles. Results of this study will be helpful to judiciously choose parents in apple breeding programs to improve compatibility. Springer Science+Business Media B.V., 2011 Malus domestica nationallicence Apple breeding nationallicence Pollinator cultivars nationallicence Apple production nationallicence de Albuquerque Jr. Celso Universidade do Sul de Santa Catarina, Av. José Acácio Moreira, no. 787, C.P. 370, CEP: 88704-900, Tubarão, SC, Brazil aut Denardi Frederico Epagri, E.E. Caçador, C.P. 591, CEP 89500-000, Caçador, SC, Brazil aut de Mesquita Dantas Adriana Universidade Estadual do Rio Grande do Sul, Rua Benjamin Constant, 229, 95700-000, Bento Gonçalves, RS, Brazil aut Nodari Rubens Programa de pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, C.P. 476, CEP 88040-900, Florianópolis, SC, Brazil aut Euphytica Springer Netherlands 181/2(2011-09-01), 277-284 0014-2336 181:2<277 2011 181 10681 https://doi.org/10.1007/s10681-011-0431-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0431-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Albuquerque Jr Celso Universidade do Sul de Santa Catarina, Av. José Acácio Moreira, no. 787, C.P. 370, CEP: 88704-900, Tubarão, SC, Brazil aut NATIONALLICENCE 700 1- Denardi Frederico Epagri, E.E. Caçador, C.P. 591, CEP 89500-000, Caçador, SC, Brazil aut NATIONALLICENCE 700 1- de Mesquita Dantas Adriana Universidade Estadual do Rio Grande do Sul, Rua Benjamin Constant, 229, 95700-000, Bento Gonçalves, RS, Brazil aut NATIONALLICENCE 700 1- Nodari Rubens Programa de pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, C.P. 476, CEP 88040-900, Florianópolis, SC, Brazil aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 277-284 0014-2336 181:2<277 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer