caa a22 4500 445324376 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0393-2 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0393-2 Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae [Elektronische Daten] [Daisuke Nakazawa, Tomonari Kishimoto, Takeru Sato, Tomoka Saito, Junji Amano, Sachiko Kuwayama, Hajime Okuno, Toshinari Godo, Yusuke Watanabe, Dong-Sheng Han, Masaru Nakano] Some intergeneric hybrids produced by the crosses among several colchicaceous ornamentals, Gloriosa superba ‘Lutea' (2n=2x=22), G. ‘Marron Gold' (2n=4x=44), G. ‘Verschild' (2n=7x=77), Littonia modesta Hook. (2n=2x=22), and Sandersonia aurantiaca Hook. (2n=2x=24), were subjected to genomic in situ hybridization (GISH) analysis in order to clarify their genome constitutions. Chromosome preparation was made from root tip cells of L. modesta×G. superba ‘Lutea', L. modesta×S. aurantiaca, S. aurantiaca×G. superba ‘Lutea', L. modesta×G. ‘Marron Gold', S. aurantiaca×G. ‘Marron Gold' and G. ‘Verschild'×S. aurantiaca. Total DNA of one parent was labeled with digoxigenin or biotin and used as probe, and chromosomes were counterstained with 4′-6-diamidono-2-phenylindole (DAPI). For all the nine intergeneric hybrids, chromosomes from each parent could be clearly distinguished by GISH analysis. Thus GISH analysis is a powerful tool for identifying the genome constitution of intergeneric hybrids in colchicaceous ornamentals. The results obtained by GISH analysis study may be important for further progress in breeding of colchicaceous ornamentals. Springer Science+Business Media B.V., 2011 Flow cytometry nationallicence Genome constitution nationallicence Gloriosa spp nationallicence Littonia modesta nationallicence Sandersonia aurantiaca nationallicence Nakazawa Daisuke Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Kishimoto Tomonari Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Sato Takeru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Saito Tomoka Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Amano Junji Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Kuwayama Sachiko Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Okuno Hajime 3250-201 Fukaisawa-machi, Naka-ku, 599-8236, Sakai, Japan aut Godo Toshinari Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-machi, 939-2713, Toyama, Japan aut Watanabe Yusuke Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Han Dong-Sheng Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Nakano Masaru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Euphytica Springer Netherlands 181/2(2011-09-01), 197-202 0014-2336 181:2<197 2011 181 10681 https://doi.org/10.1007/s10681-011-0393-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0393-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakazawa Daisuke Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Kishimoto Tomonari Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Sato Takeru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Saito Tomoka Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Amano Junji Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Kuwayama Sachiko Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Okuno Hajime 3250-201 Fukaisawa-machi, Naka-ku, 599-8236, Sakai, Japan aut NATIONALLICENCE 700 1- Godo Toshinari Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-machi, 939-2713, Toyama, Japan aut NATIONALLICENCE 700 1- Watanabe Yusuke Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Han Dong-Sheng Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Nakano Masaru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 197-202 0014-2336 181:2<197 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer