caa a22 4500 445324384 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0426-x doi (NATIONALLICENCE)springer-10.1007/s10681-011-0426-x Molecular S -genotyping and determination of S - RNase -based incompatibility groups in loquat [ Eriobotrya japonica (Thunb.) Lindl [Elektronische Daten] ] [Laura Carrera, Javier Sanzol, Esteve Soler, María Herrero, Jose Hormaza] Loquat (Eriobotrya japonica Lindl.; Pyrinae, Rosaceae) is an evergreen fruit tree which is acquiring increasing importance as an alternative crop in warm-temperate and subtropical regions. Recent pollination studies suggest that crop intensification has resulted in a reduction of fruit productivity in different locations. Loquat exhibits gametophytic self-incompatibility (GSI) and, consequently, fruit set strongly depends on cross-pollination between intercompatible cultivars. S-genotyping and incompatibility group assignment is therefore essential for adequate loquat orchard design. This study reports the molecular characterization of the S-RNase allele composition of 31 loquat cultivars grown in Spain. PCR with primers developed from loquat S-RNase sequences was used in combination with high-performance capillary polyacrilamide electrophoresis for the fine-evaluation of length polymorphisms at the S-RNase gene. This approach allowed to uncover a new S-RNase allele (S 5), whose sequence is reported and to characterize the first six S-RNase-based incompatibility groups (I-VI) in loquat, resulting from the combination of five S-alleles (S 1-S 5). The large number of cultivars that could be assigned to only six groups suggests a narrow genetic base for this crop in Spain and stresses the need for careful determination of the S-genotype of cultivars in an attempt to overcome production problems associated with inadequate pollination. Springer Science+Business Media B.V., 2011 Eriobotrya japonica nationallicence Gametophytic self-incompatibility nationallicence Loquat nationallicence S-RNase nationallicence S -genotype nationallicence Carrera Laura Departamento de Pomología, Estación Experimental de Aula Dei - CSIC, Apdo. 13034, 50080, Zaragoza, Spain aut Sanzol Javier Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. de Montañana 930, 50059, Zaragoza, Spain aut Soler Esteve Cooperativa de Callosa d'en Sarrià, 03510, Alicante, Spain aut Herrero María Departamento de Pomología, Estación Experimental de Aula Dei - CSIC, Apdo. 13034, 50080, Zaragoza, Spain aut Hormaza Jose Instituto de Hortofruticultura Subtropical y Mediterránea ‘La Mayora' (IHSM-UMA-CSIC), Estación Experimental La Mayora, Consejo Superior de Investigaciones Científicas, 29750, Algarrobo-Costa, Málaga, Spain aut Euphytica Springer Netherlands 181/2(2011-09-01), 267-275 0014-2336 181:2<267 2011 181 10681 https://doi.org/10.1007/s10681-011-0426-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0426-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carrera Laura Departamento de Pomología, Estación Experimental de Aula Dei - CSIC, Apdo. 13034, 50080, Zaragoza, Spain aut NATIONALLICENCE 700 1- Sanzol Javier Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. de Montañana 930, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Soler Esteve Cooperativa de Callosa d'en Sarrià, 03510, Alicante, Spain aut NATIONALLICENCE 700 1- Herrero María Departamento de Pomología, Estación Experimental de Aula Dei - CSIC, Apdo. 13034, 50080, Zaragoza, Spain aut NATIONALLICENCE 700 1- Hormaza Jose Instituto de Hortofruticultura Subtropical y Mediterránea ‘La Mayora' (IHSM-UMA-CSIC), Estación Experimental La Mayora, Consejo Superior de Investigaciones Científicas, 29750, Algarrobo-Costa, Málaga, Spain aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 267-275 0014-2336 181:2<267 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer