caa a22 4500 445324392 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0419-9 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0419-9 Development and application of a SRAP marker for the identification of sex in Buchloe dactyloides [Elektronische Daten] [Ying-jie Zhou, Xian-guo Wang, Xin-quan Zhang] Buffalograss, Buchloe dactyloides (Nutt.) Engelm, is a dioecious turfgrass native to the Great Plains of North America. Since its naturalization, it has become the most wildly cultivated warm-season turfgrass in northern China. While dioecious plants represent only a small proportion of all plant species, they are important models in the study of plant sex determination and evolution. The identification of the sexes is important in the theory and practice of breeding programs. At present, there is no effective method to determine the sex of early stage buffalograss. The objective of this study was to use sequence-related amplified polymorphism (SRAP) and integrated bulked segregant analysis (BSA) technology to find sex linked markers in B. dactyloides. A total of 228 primer combinations were screened and 2,690 SRAP bands examined. Only ME9/EM2 could generate a specific fragment (~240bp) in all the female plants, which was absent in male plants. The methods described here provide a simple and reproducible means of early sex identification in B. dactyloides. Springer Science+Business Media B.V., 2011 Buchloe dactyloides nationallicence Dioecious nationallicence Sex identification nationallicence Sequence-related amplified polymorphism nationallicence Bulked segregant analysis nationallicence Zhou Ying-jie Department Grassland Science, Sichuan Agricultural University, 625014, Yaan, China aut Wang Xian-guo Institute of Grassland Science, China Agricultural University, 100193, Beijing, China aut Zhang Xin-quan Department Grassland Science, Sichuan Agricultural University, 625014, Yaan, China aut Euphytica Springer Netherlands 181/2(2011-09-01), 261-266 0014-2336 181:2<261 2011 181 10681 https://doi.org/10.1007/s10681-011-0419-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0419-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Ying-jie Department Grassland Science, Sichuan Agricultural University, 625014, Yaan, China aut NATIONALLICENCE 700 1- Wang Xian-guo Institute of Grassland Science, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhang Xin-quan Department Grassland Science, Sichuan Agricultural University, 625014, Yaan, China aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 261-266 0014-2336 181:2<261 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer