caa a22 4500 445324414 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10681-011-0438-6 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0438-6 Genome constitution of Narcissus variety, ‘Tête-à-Tête', analysed through GISH and NBS profiling [Elektronische Daten] [Hongzhi Wu, Munikote Ramanna, Paul Arens, Jaap van Tuyl] The Narcissus variety, ‘Tête-à-Tête', has been the most popular variety since 1949, and a well known allotriploid (2n=3x=24+B) of spontaneous origin. Because the identity of one of the parents of this variety was uncertain, the genome constitution of ‘Tête-à-Tête' was investigated by using genomic in situ hybridization (GISH) and NBS profiling. Both of these techniques confirmed that two different species of Narcissus, viz., N. tazetta (2n=2x=20) and N. cyclamineus (2n=2x=14) are the parents. GISH clearly identified 10 chromosomes of N. tazetta and 14 chromosomes of N. cyclamineus, the former has contributed one and the latter has contributed two genomes. One B chromosome was identically labelled as those of N. cyclamineus indicating the affinity of the special chromosome to this species. Due to its male and female sterility ‘Tête-à-Tête' is unsuitable as a parent for utilizing in further breeding, it might be possible to re-synthesize a ‘Tête-à-Tête' like variety using the now known parents and the original pathway. Springer Science+Business Media B.V., 2011 Genome constitution nationallicence Narcissus nationallicence Daffodil nationallicence Tête-à-Tête nationallicence GISH nationallicence NBS profiling nationallicence Wu Hongzhi College of Landscape and Horticulture, Yunnan Agricultural University, 650201, Kunming, China aut Ramanna Munikote Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut Arens Paul Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut van Tuyl Jaap Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut Euphytica Springer Netherlands 181/2(2011-09-01), 285-292 0014-2336 181:2<285 2011 181 10681 https://doi.org/10.1007/s10681-011-0438-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0438-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Hongzhi College of Landscape and Horticulture, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 700 1- Ramanna Munikote Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Arens Paul Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Tuyl Jaap Plant Breeding, Wageningen University and Research Center, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 181/2(2011-09-01), 285-292 0014-2336 181:2<285 2011 181 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer