caa a22 4500 445324538 CHVBK 20180317142715.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10681-011-0398-x doi (NATIONALLICENCE)springer-10.1007/s10681-011-0398-x A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers [Elektronische Daten] [Farzaneh Razavi, Ellen De Keyser, Jan De Riek, Marie Van Labeke] Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria × ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp. Springer Science+Business Media B.V., 2011 Fragaria × ananassa nationallicence Fragaria chiloensis nationallicence Fragaria vesca nationallicence Strawberry cultivar nationallicence Drought stress nationallicence Water deficit nationallicence AFLP markers nationallicence EST marker nationallicence Genetic relationship nationallicence Clustering analysis nationallicence Razavi Farzaneh Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut De Keyser Ellen ILVO, Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium aut De Riek Jan ILVO, Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium aut Van Labeke Marie Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Euphytica Springer Netherlands 180/3(2011-08-01), 385-409 0014-2336 180:3<385 2011 180 10681 https://doi.org/10.1007/s10681-011-0398-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0398-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Razavi Farzaneh Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- De Keyser Ellen ILVO, Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- De Riek Jan ILVO, Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Van Labeke Marie Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/3(2011-08-01), 385-409 0014-2336 180:3<385 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer