caa a22 4500 445324570 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10681-011-0381-6 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0381-6 Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato [Elektronische Daten] [Kazuyuki Mori, Yu Sakamoto, Nobuhiro Mukojima, Seiji Tamiya, Takashi Nakao, Takashige Ishii, Kazuyoshi Hosaka] Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry chc) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5h after DNA extraction, which is a third of the operation time compared with separate PCR reactions for each marker. Springer Science+Business Media B.V., 2011 Multiplex PCR nationallicence Potato nationallicence Cyst nematode nationallicence Potato virus X nationallicence Potato virus Y nationallicence Late blight nationallicence Mori Kazuyuki Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut Sakamoto Yu Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut Mukojima Nobuhiro Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut Tamiya Seiji National Agricultural Research Center for Hokkaido Region, Memuro, Kasai, 082-0081, Hokkaido, Japan aut Nakao Takashi Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut Ishii Takashige Graduate School of Agricultural Science, Kobe University, Nada, 657-8501, Kobe, Hyogo, Japan aut Hosaka Kazuyoshi National Agricultural Research Center for Hokkaido Region, Memuro, Kasai, 082-0081, Hokkaido, Japan aut Euphytica Springer Netherlands 180/3(2011-08-01), 347-355 0014-2336 180:3<347 2011 180 10681 https://doi.org/10.1007/s10681-011-0381-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0381-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mori Kazuyuki Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut NATIONALLICENCE 700 1- Sakamoto Yu Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut NATIONALLICENCE 700 1- Mukojima Nobuhiro Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut NATIONALLICENCE 700 1- Tamiya Seiji National Agricultural Research Center for Hokkaido Region, Memuro, Kasai, 082-0081, Hokkaido, Japan aut NATIONALLICENCE 700 1- Nakao Takashi Nagasaki Agricultural and Forestry Technical Development Center, Unzen, 854-0302, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ishii Takashige Graduate School of Agricultural Science, Kobe University, Nada, 657-8501, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Hosaka Kazuyoshi National Agricultural Research Center for Hokkaido Region, Memuro, Kasai, 082-0081, Hokkaido, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/3(2011-08-01), 347-355 0014-2336 180:3<347 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer