caa a22 4500 445324597 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10681-011-0403-4 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0403-4 QTL mapping of powdery mildew susceptibility in hop ( Humulus lupulus L.) [Elektronische Daten] [John Henning, M. Townsend, David Gent, Nahla Bassil, Paul Matthews, Emily Buck, Ron Beatson] Hop powdery mildew [Podosphaera macularis (Wallr.) U. Braun & S. Takam.] is best controlled via the production of resistant varieties. Recent evidence supports selection against plant susceptibility genes to fungal pathogens as a more durable resistance mechanism than selection for resistance genes. The objective of this study was to identify molecular-based QTLs, their genetic effects and epistasis among QTLs associated with susceptibility to powdery mildew. Parents and offspring from the cross, ‘Perle'בUSDA 19058M', were clonally replicated and inoculated in a greenhouse using a CRD experimental design in Corvallis, OR. DNA was extracted, purified and analyzed via three different marker systems. Analysis of the resulting markers was based upon the "two-way pseudo-testcross” procedure. QTL mapping using multiple interval mapping and Bayesian interval mapping analyses were performed using WinQTL Cartographer 2.5_003. Comparison amongst mapping analyses identified three persistent QTLs on three linkage groups without significant epistatic effect upon expression. The persistent QTL on linkage group C7 had both additive and dominant effects controlling phenotype expression. The presence or absence of the two AFLP markers bordering the QTL on C7 defined susceptibility in offspring. This is the first report in hop identifying molecular markers linked to QTLs associated with disease susceptibility. Springer Science+Business Media B.V., 2011 AFLP nationallicence DArT nationallicence Humulus lupulus nationallicence Microsatellite nationallicence Powdery mildew nationallicence QTL mapping nationallicence S-genes nationallicence Henning John USDA-ARS-Forage Seed Crop Research Unit, 3450 SW Campus Way, 97331, Corvallis, OR, USA aut Townsend M. Department of Crop & Soil Sciences, Oregon State University, 97331, Corvallis, OR, USA aut Gent David USDA-ARS-Forage Seed Crop Research Unit, 3450 SW Campus Way, 97331, Corvallis, OR, USA aut Bassil Nahla USDA-ARS-NCGR, 33447 Peoria Rd, 97333, Corvallis, OR, USA aut Matthews Paul Hopsteiner, S. S. Steiner, 655 Madison Ave, 10065, New York, NY, USA aut Buck Emily Plant & Food Research, Auckland Mail Centre, Private Bag 92169, 1142, Auckland, New Zealand aut Beatson Ron Plant & Food Research, Auckland Mail Centre, Private Bag 92169, 1142, Auckland, New Zealand aut Euphytica Springer Netherlands 180/3(2011-08-01), 411-420 0014-2336 180:3<411 2011 180 10681 https://doi.org/10.1007/s10681-011-0403-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0403-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henning John USDA-ARS-Forage Seed Crop Research Unit, 3450 SW Campus Way, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Townsend M. Department of Crop & Soil Sciences, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Gent David USDA-ARS-Forage Seed Crop Research Unit, 3450 SW Campus Way, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Bassil Nahla USDA-ARS-NCGR, 33447 Peoria Rd, 97333, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Matthews Paul Hopsteiner, S. S. Steiner, 655 Madison Ave, 10065, New York, NY, USA aut NATIONALLICENCE 700 1- Buck Emily Plant & Food Research, Auckland Mail Centre, Private Bag 92169, 1142, Auckland, New Zealand aut NATIONALLICENCE 700 1- Beatson Ron Plant & Food Research, Auckland Mail Centre, Private Bag 92169, 1142, Auckland, New Zealand aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/3(2011-08-01), 411-420 0014-2336 180:3<411 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer