caa a22 4500 445324619 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10681-011-0417-y doi (NATIONALLICENCE)springer-10.1007/s10681-011-0417-y Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce ( Lactuca sativa L.) [Elektronische Daten] [M. Hayashi, A. Ujiie, H. Serizawa, H. Sassa, H. Kakui, T. Oda, T. Koba] Genetic male sterility (GMS) has been a useful system for the production of hybrid varieties in self-pollinated plants. We obtained a GMS line developed from a spontaneous mutation in lettuce (Lactuca sativa L.). Genetic analysis in our previous study revealed that the sterility was controlled by a recessive gene which was named ms-S. For simple and quick screening of individuals showing male sterility, we attempted molecular mapping of the ms-S locus using an amplified fragment length polymorphism (AFLP) technique. From the examination of 4,096 AFLP primer combinations, 63 AFLP markers were found to be linked to the gene and nine of them were successfully converted into sequence characterized amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers. Linkage analysis indicated that these nine markers were closely linked to the ms-S gene and all were located on the same side of the gene. The minimum genetic distance between the ms-S gene and a marker was 3.1cM. These results provide additional information for map-based cloning of the ms-S gene and will be of great help for lettuce breeding using GMS to produce F1 hybrids. Springer Science+Business Media B.V., 2011 AFLP nationallicence CAPS marker nationallicence Genetic male sterility nationallicence Lettuce nationallicence SCAR marker nationallicence Hayashi M. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Ujiie A. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Serizawa H. Nagano Vegetable and Ornamental Crops Experimental Station, 1066-1 Aza Tokoo, Oaza Souga, 399-6461, Siojiri City, Nagano, Japan aut Sassa H. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Kakui H. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Oda T. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Koba T. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Euphytica Springer Netherlands 180/3(2011-08-01), 429-436 0014-2336 180:3<429 2011 180 10681 https://doi.org/10.1007/s10681-011-0417-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0417-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayashi M. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Ujiie A. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Serizawa H. Nagano Vegetable and Ornamental Crops Experimental Station, 1066-1 Aza Tokoo, Oaza Souga, 399-6461, Siojiri City, Nagano, Japan aut NATIONALLICENCE 700 1- Sassa H. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Kakui H. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Oda T. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Koba T. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/3(2011-08-01), 429-436 0014-2336 180:3<429 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer