caa a22 4500 445324724 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10681-011-0383-4 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0383-4 Development of a PCR assay and marker-assisted transfer of leaf rust resistance gene Lr58 into adapted winter wheats [Elektronische Daten] [Vasu Kuraparthy, Shilpa Sood, Gina-Brown Guedira, Bikram Gill] Leaf rust resistance gene Lr58 derived from Aegilops triuncialis L. was transferred to the hard red winter wheat (HRWW) cultivars Jagger and Overley by standard backcrossing and marker-assisted selection (MAS). A co-dominant PCR-based sequence tagged site (STS) marker was developed based on the sequence information of the RFLP marker (XksuH16) diagnostically detecting the alien segment in T2BS·2BL-2tL(0.95). STS marker Xncw-Lr58-1 was used to select backcross F1 plants with rust resistance. The co-dominant marker polymorphism detected by primer pair NCW-Lr58-1 efficiently identified the homozygous BC3F2 plants with rust resistance gene Lr58. The STS marker Xncw-Lr58-1 showed consistent diagnostic polymorphism between the resistant source and the wheat cultivars selected by the US Wheat Coordinated Agricultural Project. The utility and compatibility of the STS marker in MAS programs involving robust genotyping platforms was demonstrated in both agarose-based and capillary-based platforms. Screening backcross derivatives carrying Lr58 with various rust races at seedling stage suggested the transferred rust resistance in adapted winter wheats is stable in both cultivar backgrounds. Lr58 in adapted winter wheat backgrounds could be used in combination with other resistance genes in wheat rust resistance breeding. Springer Science+Business Media B.V., 2011 Wheat nationallicence Rust resistance nationallicence Marker-assisted selection nationallicence Mapping nationallicence MAS : Marker assisted selection nationallicence STS : Sequence tagged sites nationallicence RFLP : Restriction fragment length polymorphism nationallicence HRWW : Hard red winter wheat nationallicence CAP : Co-ordinated agricultural project nationallicence Kuraparthy Vasu Crop Science Department, North Carolina State University, 27695, Raleigh, NC, USA aut Sood Shilpa Crop Science Department, North Carolina State University, 27695, Raleigh, NC, USA aut Guedira Gina-Brown USDA-ARS, Regional Small Grains Genotyping Laboratory, North Carolina State University, 27695, Raleigh, NC, USA aut Gill Bikram Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, 66506-5502, Manhattan, KS, USA aut Euphytica Springer Netherlands 180/2(2011-07-01), 227-234 0014-2336 180:2<227 2011 180 10681 https://doi.org/10.1007/s10681-011-0383-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0383-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuraparthy Vasu Crop Science Department, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Sood Shilpa Crop Science Department, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Guedira Gina-Brown USDA-ARS, Regional Small Grains Genotyping Laboratory, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Gill Bikram Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, 66506-5502, Manhattan, KS, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/2(2011-07-01), 227-234 0014-2336 180:2<227 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer