caa a22 4500 445324740 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10681-010-0334-5 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0334-5 Combining ability, heterosis and genetic diversity in tropical maize ( Zea mays L.) under stress and non-stress conditions [Elektronische Daten] [Dan Makumbi, Javier Betrán, Marianne Bänziger, Jean-Marcel Ribaut] Drought and low soil fertility are considered the most important abiotic stresses limiting maize production in sub-Saharan Africa. Knowledge of the combining ability and diversity of inbred lines with tolerance to the two stresses and for those used as testers would be beneficial in setting breeding strategies for stress and nonstress environments. We used 15 tropical maize inbred lines to (i) evaluate the combining ability for grain yield (GY), (ii) assess the genetic diversity of this set of inbred lines using RFLP, SSR, and AFLP markers, (iii) estimate heterosis and assess the relationship between F1 hybrid performance, genetic diversity and heterosis, and (iv) assess genotype×environment interaction of inbred lines and their hybrids. The F1 diallel hybrids and parental inbreds were evaluated under drought stress, low N stress, and well-watered conditions at six locations in three countries. General combining ability (GCA) effects were highly significant (P<0.01) for GY across stresses and well-watered environments. Inbred lines CML258, CML339, CML341, and CML343 had the best GCA effects for GY across environments. Additive genetic effects were more important for GY under drought stress and well-watered conditions but not under low N stress, suggesting different gene action in control of GY. Clustering based on genetic distance (GD) calculated using combined marker data grouped lines according to pedigree. Positive correlation was found between midparent heterosis (MPH) and specific combining ability (SCA), GD and GY. Hybrid breeding program targeting stress environments would benefit from the accumulation of favorable alleles for drought tolerance in both parental lines. Springer Science+Business Media B.V., 2011 Abiotic stress nationallicence Combining ability nationallicence Diversity nationallicence Drought nationallicence Maize nationallicence Nitrogen nationallicence Makumbi Dan International Maize and Wheat Improvement Center (CIMMYT), P.O. Box 1041-00621, Nairobi, Kenya aut Betrán Javier Syngenta Seeds S.A.S., 12 Chemin de L'Hobit, 31790, Saint-Saveur, France aut Bänziger Marianne International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, México D.F., México aut Ribaut Jean-Marcel Generation Challenge Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, México D.F., México aut Euphytica Springer Netherlands 180/2(2011-07-01), 143-162 0014-2336 180:2<143 2011 180 10681 https://doi.org/10.1007/s10681-010-0334-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0334-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Makumbi Dan International Maize and Wheat Improvement Center (CIMMYT), P.O. Box 1041-00621, Nairobi, Kenya aut NATIONALLICENCE 700 1- Betrán Javier Syngenta Seeds S.A.S., 12 Chemin de L'Hobit, 31790, Saint-Saveur, France aut NATIONALLICENCE 700 1- Bänziger Marianne International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, México D.F., México aut NATIONALLICENCE 700 1- Ribaut Jean-Marcel Generation Challenge Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, México D.F., México aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/2(2011-07-01), 143-162 0014-2336 180:2<143 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer