caa a22 4500 445324767 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10681-011-0376-3 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0376-3 Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize [Elektronische Daten] [Aida Kebede, Baldev Dhillon, Wolfgang Schipprack, Jose Araus, Marianne Bänziger, Kassa Semagn, Gregorio Alvarado, Albrecht Melchinger] For in vivo production of doubled haploid (DH) lines in maize, the rate of haploid induction is of crucial importance. Maternal haploid induction depends primarily on the inducer used as a pollinator. However, the source germplasm used as a maternal parent and the environmental conditions for induction may also influence haploid induction and these aspects have not been examined in tropical maize so far. The objectives of our study were to (i) monitor the variation for haploid induction rate (HIR) among diverse source germplasm in tropical maize, (ii) determine the relative importance of general (GCA) and specific (SCA) combining abilities for HIR, and (iii) investigate the influence of summer and winter seasons and genotype×season interactions on this trait. Ten inbreds were mated in a half diallel design. The resulting 45 F1 single crosses were pollinated with the haploid inducer hybrid RWS×UH400 during the summer 2008 and winter 2009 seasons in a lowland tropical environment in Mexico. HIR of the single crosses averaged over seasons ranged from 2.90 to 9.66% with an overall mean of 6.74%. Mean HIR was significantly (P<0.01) higher during the winter (7.37%) than summer season (6.11%). Significant (P<0.01) variation was observed due to GCA effects of parental inbreds of single crosses but not for SCA, GCA×season and SCA×season interactions. Our study underpins that a higher HIR in tropical maize can be obtained by selecting appropriate source germplasm and undertaking pollination under favorable environmental conditions. Springer Science+Business Media B.V., 2011 Haploid induction rate nationallicence Tropical source germplasm nationallicence Diallel crosses nationallicence General combining ability nationallicence Season nationallicence Maize nationallicence Kebede Aida International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut Dhillon Baldev Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut Schipprack Wolfgang Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut Araus Jose Department of Vegetal Biology, University of Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain aut Bänziger Marianne International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut Semagn Kassa International Maize and Wheat Improvement Center (CIMMYT), Village Market, P.O. Box 1041, 00621, Nairobi, Kenya aut Alvarado Gregorio International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut Melchinger Albrecht Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut Euphytica Springer Netherlands 180/2(2011-07-01), 219-226 0014-2336 180:2<219 2011 180 10681 https://doi.org/10.1007/s10681-011-0376-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0376-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kebede Aida International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Dhillon Baldev Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Schipprack Wolfgang Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Araus Jose Department of Vegetal Biology, University of Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Bänziger Marianne International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Semagn Kassa International Maize and Wheat Improvement Center (CIMMYT), Village Market, P.O. Box 1041, 00621, Nairobi, Kenya aut NATIONALLICENCE 700 1- Alvarado Gregorio International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Melchinger Albrecht Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/2(2011-07-01), 219-226 0014-2336 180:2<219 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer