caa a22 4500 445324775 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10681-011-0405-2 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0405-2 A novel blast resistance locus in a rice ( Oryza sativa L.) cultivar, Chumroo, of Bhutan [Elektronische Daten] [Kei Matsushita, Nobuko Yasuda, Thinlay,, Shinzo Koizumi, Taketo Ashizawa, Yoshihiro Sunohara, Shuichi Iida, Osamu Ideta, Hideo Maeda, Yoshikatsu Fujita] The rice cultivar ‘Chumroo' is commonly cultivated in the mid- and high-altitude areas of Bhutan. This cultivar has shown durable blast resistance in that area, without evidence of breakdown, for over 20years. Chumroo was inoculated with 22 blast isolates selected from the race differential standard set of Japan. The cultivar showed resistance to all the isolates. To identify the resistance gene(s), Chumroo was crossed with a susceptible rice cultivar, Koshihikari. The F1 plants of the cross showed resistance. Segregation analyses of 300 F3 family lines fitted the segregation ratio of 1:2:1 and indicated that a single dominant gene controls the resistance to a blast isolate Ao 92-06-2 (race 337.1). The Chumroo resistance locus (termed Pi46(t)) was mapped between two SSR markers, RM6748 and RM5473, on the terminal region of the long arm of chromosome 4, using linkage analysis with SSR markers. The nearest marker, RM5473, was linked to the putative resistance locus at a map distance of 3.2cM. At the chromosomal region, no true resistance genes were identified, whereas two field resistance genes were present. Therefore, we designated Pi46(t) as a novel blast resistance locus. Springer Science+Business Media B.V., 2011 Rice ( Oryza sativa L.) nationallicence Rice blast fungus ( Magnaporthe oryzae ) nationallicence Resistance gene nationallicence Genetic mapping nationallicence SSR marker nationallicence Matsushita Kei National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut Yasuda Nobuko National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut Thinlay, National Plant Protection Center, Simtokha, P.O. Box 670, Thimphu, Bhutan aut Koizumi Shinzo National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut Ashizawa Taketo National Agricultural Research Center for Tohoku Region, 3 Shimo-furumichi, Yotsuya, 014-0102, Daisen, Akita, Japan aut Sunohara Yoshihiro National Institute of Crop Science, 2-1-18 Kannondai, 305-8518, Tsukuba, Ibaraki, Japan aut Iida Shuichi National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut Ideta Osamu National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut Maeda Hideo National Institute of Crop Science, 2-1-18 Kannondai, 305-8518, Tsukuba, Ibaraki, Japan aut Fujita Yoshikatsu National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut Euphytica Springer Netherlands 180/2(2011-07-01), 273-280 0014-2336 180:2<273 2011 180 10681 https://doi.org/10.1007/s10681-011-0405-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0405-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsushita Kei National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 700 1- Yasuda Nobuko National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Thinlay National Plant Protection Center, Simtokha, P.O. Box 670, Thimphu, Bhutan aut NATIONALLICENCE 700 1- Koizumi Shinzo National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ashizawa Taketo National Agricultural Research Center for Tohoku Region, 3 Shimo-furumichi, Yotsuya, 014-0102, Daisen, Akita, Japan aut NATIONALLICENCE 700 1- Sunohara Yoshihiro National Institute of Crop Science, 2-1-18 Kannondai, 305-8518, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Iida Shuichi National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 700 1- Ideta Osamu National Agricultural Research Center for Western Region, 6-12-1 Nishi-Fukatsu, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 700 1- Maeda Hideo National Institute of Crop Science, 2-1-18 Kannondai, 305-8518, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Fujita Yoshikatsu National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 180/2(2011-07-01), 273-280 0014-2336 180:2<273 2011 180 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer