caa a22 4500 445324805 CHVBK 20180317142716.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10858-011-9484-6 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9484-6 NMR-based stable isotope resolved metabolomics in systems biochemistry [Elektronische Daten] [Teresa Fan, Andrew Lane] An important goal of metabolomics is to characterize the changes in metabolic networks in cells or various tissues of an organism in response to external perturbations or pathologies. The profiling of metabolites and their steady state concentrations does not directly provide information regarding the architecture and fluxes through metabolic networks. This requires tracer approaches. NMR is especially powerful as it can be used not only to identify and quantify metabolites in an unfractionated mixture such as biofluids or crude cell/tissue extracts, but also determine the positional isotopomer distributions of metabolites derived from a precursor enriched in stable isotopes such as 13C and 15N via metabolic transformations. In this article we demonstrate the application of a variety of 2-D NMR editing experiments to define the positional isotopomers of compounds present in polar and non-polar extracts of human lung cancer cells grown in either [U-13C]-glucose or [U-13C,15N]-glutamine as source tracers. The information provided by such experiments enabled unambiguous reconstruction of metabolic pathways, which is the foundation for further metabolic flux modeling. Springer Science+Business Media B.V., 2011 Stable isotope resolved metabolomics nationallicence Isotope editing nationallicence Fan Teresa Department of Chemistry, University of Louisville, Louisville, KY, USA aut Lane Andrew Center for Regulatory Environmental Analytical Metabolomics, University of Louisville, Louisville, KY, USA aut Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 267-280 0925-2738 49:3-4<267 2011 49 10858 https://doi.org/10.1007/s10858-011-9484-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9484-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fan Teresa Department of Chemistry, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 700 1- Lane Andrew Center for Regulatory Environmental Analytical Metabolomics, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 267-280 0925-2738 49:3-4<267 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer