caa a22 4500 445324872 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10858-011-9485-5 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9485-5 Metabolic profiling of vitamin C deficiency in Gulo − [Elektronische Daten] − mice using proton NMR spectroscopy / [Gavin Duggan, B. Joan Miller, Frank Jirik, Hans Vogel] Nutrient deficiencies are an ongoing problem in many populations and ascorbic acid is a key vitamin whose mild or acute absence leads to a number of conditions including the famously debilitating scurvy. As such, the biochemical effects of ascorbate deficiency merit ongoing scrutiny, and the Gulo knockout mouse provides a useful model for the metabolomic examination of vitamin C deficiency. Like humans, these animals are incapable of synthesizing ascorbic acid but with dietary supplements are otherwise healthy and grow normally. In this study, all vitamin C sources were removed after weaning from the diet of Gulo−/− mice (n=7) and wild type controls (n=7) for 12weeks before collection of serum. A replicate study was performed with similar parameters but animals were harvested pre-symptomatically after 2-3weeks. The serum concentration of 50 metabolites was determined by quantitative profiling of 1D proton NMR spectra. Multivariate statistical models were used to describe metabolic changes as compared to control animals; replicate study animals were used for external validation of the resulting models. The results of the study highlight the metabolites and pathways known to require ascorbate for proper flux. Springer Science+Business Media B.V., 2011 Metabolomics nationallicence Scurvy nationallicence Ascorbic acid nationallicence Glutathione nationallicence Vitamin C nationallicence Vitamin deficiency nationallicence Duggan Gavin Biochemistry Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, T2N 1N4, Calgary, AB, Canada aut Joan Miller B. Department of Biochemistry and Molecular Biology, The McCaig Institute for Bone and Joint Health, University of Calgary, T2N 4N1, Calgary, AB, Canada aut Jirik Frank Department of Biochemistry and Molecular Biology, The McCaig Institute for Bone and Joint Health, University of Calgary, T2N 4N1, Calgary, AB, Canada aut Vogel Hans Biochemistry Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, T2N 1N4, Calgary, AB, Canada aut Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 165-173 0925-2738 49:3-4<165 2011 49 10858 https://doi.org/10.1007/s10858-011-9485-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9485-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duggan Gavin Biochemistry Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, T2N 1N4, Calgary, AB, Canada aut NATIONALLICENCE 700 1- Joan Miller B. Department of Biochemistry and Molecular Biology, The McCaig Institute for Bone and Joint Health, University of Calgary, T2N 4N1, Calgary, AB, Canada aut NATIONALLICENCE 700 1- Jirik Frank Department of Biochemistry and Molecular Biology, The McCaig Institute for Bone and Joint Health, University of Calgary, T2N 4N1, Calgary, AB, Canada aut NATIONALLICENCE 700 1- Vogel Hans Biochemistry Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, T2N 1N4, Calgary, AB, Canada aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 165-173 0925-2738 49:3-4<165 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer