caa a22 4500 445324902 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10858-011-9489-1 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9489-1 Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks [Elektronische Daten] [Patrizia Bernini, Ivano Bertini, Claudio Luchinat, Paola Nincheri, Samuele Staderini, Paola Turano] 1H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t=0−4h) between blood collection and processing and of the time from processing to freezing (up to 24h). The stability of the urine metabolic profile over time (up to 24h) at various storage temperatures was monitored as a function of the different pre-analytical treatments like pre-storage centrifugation, filtration, and addition of the bacteriostatic preservative sodium azide. Appreciable changes in the profiles, reflecting changes in the concentration of a number of metabolites, were detected and discussed in terms of chemical and enzymatic reactions for both blood and urine samples. Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking. Springer Science+Business Media B.V., 2011 Metabolomics nationallicence NMR spectroscopy nationallicence Serum nationallicence Plasma nationallicence Urine nationallicence Biobanks nationallicence Bernini Patrizia Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Bertini Ivano Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Luchinat Claudio Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Nincheri Paola FiorGen Foundation, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Staderini Samuele FiorGen Foundation, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Turano Paola Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 231-243 0925-2738 49:3-4<231 2011 49 10858 https://doi.org/10.1007/s10858-011-9489-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9489-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernini Patrizia Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Bertini Ivano Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Luchinat Claudio Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Nincheri Paola FiorGen Foundation, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Staderini Samuele FiorGen Foundation, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Turano Paola Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 231-243 0925-2738 49:3-4<231 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer