caa a22 4500 445324929 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10858-011-9488-2 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9488-2 The application of micro-coil NMR probe technology to metabolomics of urine and serum [Elektronische Daten] [John Grimes, Thomas O'Connell] Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5mm NMR probe. Sample concentration is evaluated as a means to leverage the greatly improved mass sensitivity of the micro-coil probes. With very small sample volumes, the sensitivity of the micro-coil probe does indeed provide a significant advantage over the standard probe. Concentrating the samples does improve the signal detection, but the benefits do not follow the expected linear increase and are both matrix and metabolite specific. Absolute quantitation will be affected by concentration, but an analysis of relative concentrations is still possible. The choice of the micro-coil probe over a standard tube based probe will depend upon a number of factors including number of samples and initial volume but this study demonstrates the feasibility of high-throughput metabolomics with the micro-probe platform. Springer Science+Business Media B.V., 2011 Metabolomics nationallicence Micro-coil probe nationallicence High-throughput nationallicence Mass-sensitivity nationallicence Biofluids nationallicence Grimes John Hamner-UNC Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA aut O'Connell Thomas Hamner-UNC Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA aut Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 297-305 0925-2738 49:3-4<297 2011 49 10858 https://doi.org/10.1007/s10858-011-9488-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9488-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grimes John Hamner-UNC Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA aut NATIONALLICENCE 700 1- O'Connell Thomas Hamner-UNC Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/3-4(2011-04-01), 297-305 0925-2738 49:3-4<297 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer