caa a22 4500 445324988 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10858-010-9461-5 doi (NATIONALLICENCE)springer-10.1007/s10858-010-9461-5 Recovering lost magnetization: polarization enhancement in biomolecular NMR [Elektronische Daten] [Adrien Favier, Bernhard Brutscher] Experimental sensitivity remains a major drawback for the application of NMR spectroscopy to fragile and low concentrated biomolecular samples. Here we describe an efficient polarization enhancement mechanism in longitudinal-relaxation enhanced fast-pulsing triple-resonance experiments. By recovering undetectable 1H polarization originating from longitudinal relaxation during the pulse sequence, the steady-state 15N polarization becomes enhanced by up to a factor of ~5 with respect to thermal equilibrium yielding significant sensitivity improvements compared to conventional schemes. The benefits of BEST-TROSY experiments at high magnetic field strength are illustrated for various protein applications, but they will be equally useful for other protonated macromolecular systems. Springer Science+Business Media B.V., 2010 BEST nationallicence Fast NMR nationallicence Longitudinal-relaxation enhancement nationallicence Protein nationallicence Sensitivity nationallicence TROSY nationallicence Favier Adrien IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027, Grenoble, France aut Brutscher Bernhard IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027, Grenoble, France aut Journal of Biomolecular NMR Springer Netherlands 49/1(2011-01-01), 9-15 0925-2738 49:1<9 2011 49 10858 https://doi.org/10.1007/s10858-010-9461-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-010-9461-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Favier Adrien IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027, Grenoble, France aut NATIONALLICENCE 700 1- Brutscher Bernhard IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027, Grenoble, France aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/1(2011-01-01), 9-15 0925-2738 49:1<9 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer