caa a22 4500 44532502X CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10858-011-9468-6 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9468-6 Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy [Elektronische Daten] [Manuel Etzkorn, Anja Böckmann, Marc Baldus] It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4kDa Crh protein, which has been shown to involve a partially unfolded intermediate state prior to aggregation. Based on a single real-time 2D 13C-13C transition spectrum, kinetic information about the refolding and aggregation step could be extracted. In addition, structural rearrangements associated with refolding are estimated and several different aggregation scenarios were compared to the experimental data. The Author(s), 2011 Aggregation nationallicence Kinetic nationallicence Solid-state NMR nationallicence Real-time spectroscopy nationallicence Crh nationallicence Etzkorn Manuel Department of NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Böckmann Anja Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS-UCBL, Université de Lyon, 7, passage du Vercors, 69367, Lyon, France aut Baldus Marc Department of NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 121-129 0925-2738 49:2<121 2011 49 10858 https://doi.org/10.1007/s10858-011-9468-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9468-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Etzkorn Manuel Department of NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Böckmann Anja Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS-UCBL, Université de Lyon, 7, passage du Vercors, 69367, Lyon, France aut NATIONALLICENCE 700 1- Baldus Marc Department of NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 121-129 0925-2738 49:2<121 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer