caa a22 4500 445325046 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10858-010-9463-3 doi (NATIONALLICENCE)springer-10.1007/s10858-010-9463-3 A simple biosynthetic method for stereospecific resonance assignment of prochiral methyl groups in proteins [Elektronische Daten] [Michael Plevin, Olivier Hamelin, Jérôme Boisbouvier, Pierre Gans] A new method for stereospecific assignment of prochiral methyl groups in proteins is presented in which protein samples are produced using U-[13C]glucose and subsaturating amounts of 2-[13C]methyl-acetolactate. The resulting non-uniform labeling pattern allows proR and proS methyl groups to be easily distinguished by their different phases in a constant-time two-dimensional 1H-13C correlation spectra. Protein samples are conveniently prepared using the same media composition as the main uniformly-labeled sample and contain higher levels of isotope-enrichment than fractional labeling approaches. This new strategy thus represents an economically-attractive, robust alternative for obtaining isotopically-encoded stereospecific NMR assignments of prochiral methyl groups. Springer Science+Business Media B.V., 2011 Isotope labeling nationallicence Stereospecific assignments nationallicence Acetolactate nationallicence Prochiral methyl groups nationallicence Plevin Michael CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut Hamelin Olivier CNRS, Laboratoire de Chimie et Biologie des Métaux, 17 avenue des martyrs, Grenoble, France aut Boisbouvier Jérôme CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut Gans Pierre CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 61-67 0925-2738 49:2<61 2011 49 10858 https://doi.org/10.1007/s10858-010-9463-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-010-9463-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Plevin Michael CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut NATIONALLICENCE 700 1- Hamelin Olivier CNRS, Laboratoire de Chimie et Biologie des Métaux, 17 avenue des martyrs, Grenoble, France aut NATIONALLICENCE 700 1- Boisbouvier Jérôme CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut NATIONALLICENCE 700 1- Gans Pierre CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 61-67 0925-2738 49:2<61 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer