caa a22 4500 445325089 CHVBK 20180317142717.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10858-011-9470-z doi (NATIONALLICENCE)springer-10.1007/s10858-011-9470-z Extension of the HA-detection based approach: (HCA)CON(CA)H and (HCA)NCO(CA)H experiments for the main-chain assignment of intrinsically disordered proteins [Elektronische Daten] [Sampo Mäntylahti, Maarit Hellman, Perttu Permi] Extensive resonance overlap exacerbates assignment of intrinsically disordered proteins (IDPs). This issue can be circumvented by utilizing 15N, 13C′ and 1HN spins, where the chemical shift dispersion is mainly dictated by the characteristics of consecutive amino acid residues. Especially 15N and 13C′ spins offer superior chemical shift dispersion in comparison to 13Cα and 13Cβ spins. However, HN-detected experiments suffer from exchange broadening of amide proton signals on IDPs especially under alkali conditions. To that end, we propose here two novel HA-detected experiments, (HCA)CON(CA)H and (HCA)NCO(CA)H and a new assignment protocol based on panoply of unidirectional HA-detected experiments that enable robust backbone assignment of IDPs also at high pH. The new approach was tested at pH 6.5 and pH 8.5 on cancer/testis antigen CT16, a 110-residue IDP, and virtually complete backbone assignment of CT16 was obtained by employing the novel HA-detected experiments together with the previously introduced iH(CA)NCO scheme. Remarkably, also those 10 N-terminal residues that remained unassigned in our earlier HN-detection based assignment approach even at pH 6.5 were now readily assigned. Moreover, theoretical calculations and experimental results suggest that overall sensitivity of the new experiments is also applicable to small or medium sized globular proteins that require alkaline conditions. Springer Science+Business Media B.V., 2011 Assignment nationallicence CT16 nationallicence HA-detection nationallicence (HCA)CON(CA)H nationallicence (HCA)NCO(CA)H nationallicence Intrinsically disordered proteins nationallicence IDP nationallicence Mäntylahti Sampo Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut Hellman Maarit Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut Permi Perttu Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 99-109 0925-2738 49:2<99 2011 49 10858 https://doi.org/10.1007/s10858-011-9470-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9470-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mäntylahti Sampo Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Hellman Maarit Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Permi Perttu Program in Structural Biology and Biophysics, NMR Laboratory, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 49/2(2011-02-01), 99-109 0925-2738 49:2<99 2011 49 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer