caa a22 4500 445325208 CHVBK 20180317142718.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10858-011-9560-y doi (NATIONALLICENCE)springer-10.1007/s10858-011-9560-y Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR [Elektronische Daten] [Fabian Peters, Mitcheell Maestre-Martinez, Andrei Leonov, Lidija Kovačič, Stefan Becker, Rolf Boelens, Christian Griesinger] Here we present Cys-Ph-TAHA, a new nonadentate lanthanide tag for the paramagnetic labelling of proteins. The tag can be easily synthesized and is stereochemically homogenous over a wide range of temperatures, yielding NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large residual dipolar couplings and pseudocontact shifts that could be measured easily and agreed very well with the protein structure. We show that Cys-Ph-TAHA can be used to label large proteins that are biochemically challenging such as the Lac repressor in a 90kDa ternary complex with DNA and inducer. The Author(s), 2011 Lanthanides nationallicence Pseudocontact shifts nationallicence Residual dipolar couplings nationallicence Paramagnetic NMR nationallicence Peters Fabian Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Maestre-Martinez Mitcheell Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Leonov Andrei Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Kovačič Lidija Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Becker Stefan Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Boelens Rolf Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Griesinger Christian Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 329-337 0925-2738 51:3<329 2011 51 10858 https://doi.org/10.1007/s10858-011-9560-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9560-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peters Fabian Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Maestre-Martinez Mitcheell Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Leonov Andrei Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Kovačič Lidija Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Becker Stefan Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Boelens Rolf Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Griesinger Christian Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 329-337 0925-2738 51:3<329 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer