caa a22 4500 445325224 CHVBK 20180317142718.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10858-011-9532-2 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9532-2 Narrowing the conformational space sampled by two-domain proteins with paramagnetic probes in both domains [Elektronische Daten] [Soumyasri Dasgupta, Xiaoyu Hu, Peter Keizers, Wei-Min Liu, Claudio Luchinat, Malini Nagulapalli, Mark Overhand, Giacomo Parigi, Luca Sgheri, Marcellus Ubbink] Calmodulin is a two-domain protein which in solution can adopt a variety of conformations upon reorientation of its domains. The maximum occurrence (MO) of a set of calmodulin conformations that are representative of the overall conformational space possibly sampled by the protein, has been calculated from the paramagnetism-based restraints. These restraints were measured after inclusion of a lanthanide binding tag in the C-terminal domain to supplement the data obtained by substitution of three paramagnetic lanthanide ions to the calcium ion in the second calcium binding loop of the N-terminal domain. The analysis shows that the availability of paramagnetic restraints arising from metal ions placed on both domains, reduces the MO of the conformations to different extents, thereby helping to identify those conformations that can be mostly sampled by the protein. Springer Science+Business Media B.V., 2011 Conformational heterogeneity nationallicence Maximum allowed probability nationallicence Maximum occurrence nationallicence Paramagnetic proteins nationallicence Paramagnetic tag nationallicence Calmodulin nationallicence Dasgupta Soumyasri Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut Hu Xiaoyu Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut Keizers Peter Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut Liu Wei-Min Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut Luchinat Claudio Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut Nagulapalli Malini Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut Overhand Mark Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut Parigi Giacomo Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut Sgheri Luca Istituto per le Applicazioni del Calcolo, Sezione di Firenze, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy aut Ubbink Marcellus Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 253-263 0925-2738 51:3<253 2011 51 10858 https://doi.org/10.1007/s10858-011-9532-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9532-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dasgupta Soumyasri Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Hu Xiaoyu Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Keizers Peter Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut NATIONALLICENCE 700 1- Liu Wei-Min Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut NATIONALLICENCE 700 1- Luchinat Claudio Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Nagulapalli Malini Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Overhand Mark Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut NATIONALLICENCE 700 1- Parigi Giacomo Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Sgheri Luca Istituto per le Applicazioni del Calcolo, Sezione di Firenze, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Ubbink Marcellus Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO box 9502, 2300 RA, Leiden, Netherlands aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 253-263 0925-2738 51:3<253 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer