caa a22 4500 445325259 CHVBK 20180317142718.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10858-011-9533-1 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9533-1 Multiplet-filtered and gradient-selected zero-quantum TROSY experiments for 13C1H3 methyl groups in proteins [Elektronische Daten] [Michelle Gill, Arthur Palmer III] Multiplet-filtered and gradient-selected heteronuclear zero-quantum coherence (gsHZQC) TROSY experiments are described for measuring 1H-13C correlations for 13CH3 methyl groups in proteins. These experiments provide improved suppression of undesirable, broad outer components of the heteronuclear zero-quantum multiplet in medium-sized proteins, or in flexible sites of larger proteins, compared to previously described HZQC sequences (Tugarinov et al. in J Am Chem Soc 126:4921-4925, 2004; Ollerenshaw et al. in J Biomol NMR 33:25-41, 2005). Hahn-echo versions of the gsHZQC experiment also are described for measuring zero- and double-quantum transverse relaxation rate constants for identification of chemical exchange broadening. Application of the proposed pulse sequences to Escherichia coli ribonuclease HI, with a molecular mass of 18kD, indicates that improved multiplet suppression is obtained without substantial loss of sensitivity. Springer Science+Business Media B.V., 2011 Methyl nationallicence TROSY nationallicence Zero-quantum nationallicence HZQC nationallicence HMQC nationallicence RNase H nationallicence Gill Michelle Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut Palmer III Arthur Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 245-251 0925-2738 51:3<245 2011 51 10858 https://doi.org/10.1007/s10858-011-9533-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9533-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gill Michelle Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut NATIONALLICENCE 700 1- Palmer III Arthur Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 245-251 0925-2738 51:3<245 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer